Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18780 and BPHYT_RS18785 overlap by 8 nucleotides BPHYT_RS18785 and BPHYT_RS18790 overlap by 7 nucleotides BPHYT_RS18790 and BPHYT_RS18795 are separated by 2 nucleotides BPHYT_RS18795 and BPHYT_RS18800 are separated by 70 nucleotides
BPHYT_RS18780: BPHYT_RS18780 - flagellar motor switch protein FliG, at 4,222,897 to 4,223,892
_RS18780
BPHYT_RS18785: BPHYT_RS18785 - flagellar assembly protein FliH, at 4,223,885 to 4,224,562
_RS18785
BPHYT_RS18790: BPHYT_RS18790 - ATP synthase, at 4,224,556 to 4,226,205
_RS18790
BPHYT_RS18795: BPHYT_RS18795 - flagellar export protein FliJ, at 4,226,208 to 4,226,657
_RS18795
BPHYT_RS18800: BPHYT_RS18800 - flagellar hook-length control protein, at 4,226,728 to 4,228,221
_RS18800
Position (kb) 4224
4225
4226
4227 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4223.581 kb on - strand, within BPHYT_RS18780 at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.884 kb on + strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4223.885 kb on - strand at 4224.398 kb on + strand, within BPHYT_RS18785 at 4225.586 kb on - strand, within BPHYT_RS18790 at 4225.807 kb on + strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.808 kb on - strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.809 kb on + strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.810 kb on - strand, within BPHYT_RS18790 at 4225.918 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.998 kb on + strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4225.999 kb on - strand, within BPHYT_RS18790 at 4226.352 kb on + strand, within BPHYT_RS18795 at 4226.353 kb on - strand, within BPHYT_RS18795 at 4226.539 kb on - strand, within BPHYT_RS18795 at 4226.727 kb on + strand at 4226.728 kb on - strand at 4227.105 kb on + strand, within BPHYT_RS18800 at 4227.106 kb on - strand, within BPHYT_RS18800
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 4,223,581 - BPHYT_RS18780 0.69 +1.7 4,223,884 + -0.1 4,223,884 + -0.4 4,223,884 + +0.6 4,223,884 + -0.9 4,223,884 + -0.0 4,223,885 - +1.2 4,223,885 - -0.8 4,223,885 - +0.4 4,223,885 - +0.5 4,223,885 - -0.9 4,223,885 - -0.5 4,223,885 - +0.2 4,223,885 - +0.1 4,223,885 - -0.2 4,223,885 - -0.6 4,223,885 - -0.8 4,224,398 + BPHYT_RS18785 0.76 +0.6 4,225,586 - BPHYT_RS18790 0.62 +2.9 4,225,807 + BPHYT_RS18790 0.76 -2.0 4,225,808 - BPHYT_RS18790 0.76 -0.6 4,225,808 - BPHYT_RS18790 0.76 -0.3 4,225,808 - BPHYT_RS18790 0.76 +0.3 4,225,809 + BPHYT_RS18790 0.76 +0.4 4,225,809 + BPHYT_RS18790 0.76 -0.3 4,225,810 - BPHYT_RS18790 0.76 -1.4 4,225,810 - BPHYT_RS18790 0.76 -0.2 4,225,918 + BPHYT_RS18790 0.83 +0.2 4,225,998 + BPHYT_RS18790 0.87 -1.0 4,225,998 + BPHYT_RS18790 0.87 -0.8 4,225,999 - BPHYT_RS18790 0.87 +0.6 4,225,999 - BPHYT_RS18790 0.87 -2.4 4,226,352 + BPHYT_RS18795 0.32 -0.7 4,226,353 - BPHYT_RS18795 0.32 +0.7 4,226,539 - BPHYT_RS18795 0.74 -0.8 4,226,727 + +0.1 4,226,728 - -0.5 4,227,105 + BPHYT_RS18800 0.25 -0.0 4,227,106 - BPHYT_RS18800 0.25 -1.4
Or see this region's nucleotide sequence