Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17795 and BPHYT_RS17800 are separated by 302 nucleotides BPHYT_RS17800 and BPHYT_RS17805 are separated by 88 nucleotides BPHYT_RS17805 and BPHYT_RS17810 are separated by 91 nucleotides
BPHYT_RS17795: BPHYT_RS17795 - short-chain dehydrogenase, at 4,016,598 to 4,017,380
_RS17795
BPHYT_RS17800: BPHYT_RS17800 - hypothetical protein, at 4,017,683 to 4,018,144
_RS17800
BPHYT_RS17805: BPHYT_RS17805 - hypothetical protein, at 4,018,233 to 4,018,874
_RS17805
BPHYT_RS17810: BPHYT_RS17810 - transcriptional regulator, at 4,018,966 to 4,019,694
_RS17810
Position (kb) 4017
4018
4019 Strain fitness (log2 ratio) -2
-1
0
1 at 4017.222 kb on - strand, within BPHYT_RS17795 at 4017.332 kb on + strand at 4017.386 kb on - strand at 4017.622 kb on + strand at 4017.637 kb on + strand at 4018.065 kb on + strand, within BPHYT_RS17800 at 4018.066 kb on - strand, within BPHYT_RS17800 at 4018.261 kb on - strand at 4018.576 kb on - strand, within BPHYT_RS17805 at 4018.674 kb on + strand, within BPHYT_RS17805 at 4018.898 kb on + strand at 4018.953 kb on + strand at 4019.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 4,017,222 - BPHYT_RS17795 0.80 +0.4 4,017,332 + -0.9 4,017,386 - -0.1 4,017,622 + -0.7 4,017,637 + -1.9 4,018,065 + BPHYT_RS17800 0.83 -0.7 4,018,066 - BPHYT_RS17800 0.83 -1.5 4,018,261 - +1.0 4,018,576 - BPHYT_RS17805 0.53 +0.6 4,018,674 + BPHYT_RS17805 0.69 -0.8 4,018,898 + +0.2 4,018,953 + -0.8 4,019,006 + +0.5
Or see this region's nucleotide sequence