Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14345 and BPHYT_RS14350 are separated by 69 nucleotides BPHYT_RS14350 and BPHYT_RS14355 are separated by 15 nucleotides BPHYT_RS14355 and BPHYT_RS14360 overlap by 4 nucleotides BPHYT_RS14360 and BPHYT_RS14365 overlap by 14 nucleotides
BPHYT_RS14345: BPHYT_RS14345 - beta-hexosaminidase, at 3,275,455 to 3,276,483
_RS14345
BPHYT_RS14350: BPHYT_RS14350 - holo-[acyl-carrier-protein] synthase, at 3,276,553 to 3,276,975
_RS14350
BPHYT_RS14355: BPHYT_RS14355 - pyridoxine 5'-phosphate synthase, at 3,276,991 to 3,277,755
_RS14355
BPHYT_RS14360: BPHYT_RS14360 - DNA repair protein RecO, at 3,277,752 to 3,278,681
_RS14360
BPHYT_RS14365: BPHYT_RS14365 - GTPase Era, at 3,278,668 to 3,279,531
_RS14365
Position (kb)
3276
3277
3278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3276.093 kb on - strand, within BPHYT_RS14345 at 3276.161 kb on + strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.549 kb on - strand at 3277.756 kb on - strand at 3277.811 kb on - strand at 3277.870 kb on + strand, within BPHYT_RS14360 at 3277.871 kb on - strand, within BPHYT_RS14360 at 3277.975 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.122 kb on + strand, within BPHYT_RS14360 at 3278.123 kb on - strand, within BPHYT_RS14360 at 3278.123 kb on - strand, within BPHYT_RS14360 at 3278.188 kb on + strand, within BPHYT_RS14360 at 3278.422 kb on + strand, within BPHYT_RS14360 at 3278.423 kb on - strand at 3278.423 kb on - strand, within BPHYT_RS14360 at 3278.426 kb on + strand, within BPHYT_RS14360 at 3278.426 kb on + strand, within BPHYT_RS14360 at 3278.427 kb on - strand, within BPHYT_RS14360 at 3278.427 kb on - strand, within BPHYT_RS14360 at 3278.570 kb on + strand, within BPHYT_RS14360 at 3278.570 kb on + strand, within BPHYT_RS14360 at 3278.571 kb on - strand, within BPHYT_RS14360 at 3278.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 3,276,093 - BPHYT_RS14345 0.62 +0.8 3,276,161 + BPHYT_RS14345 0.69 +0.3 3,276,162 - BPHYT_RS14345 0.69 -0.9 3,276,162 - BPHYT_RS14345 0.69 +0.4 3,276,162 - BPHYT_RS14345 0.69 +3.7 3,276,549 - -0.6 3,277,756 - +0.8 3,277,811 - +0.3 3,277,870 + BPHYT_RS14360 0.13 -1.8 3,277,871 - BPHYT_RS14360 0.13 +2.0 3,277,975 + BPHYT_RS14360 0.24 -1.2 3,278,122 + BPHYT_RS14360 0.40 +0.1 3,278,122 + BPHYT_RS14360 0.40 -1.1 3,278,122 + BPHYT_RS14360 0.40 -0.2 3,278,123 - BPHYT_RS14360 0.40 +0.2 3,278,123 - BPHYT_RS14360 0.40 +0.2 3,278,188 + BPHYT_RS14360 0.47 +0.7 3,278,422 + BPHYT_RS14360 0.72 +0.2 3,278,423 - -1.1 3,278,423 - BPHYT_RS14360 0.72 -1.4 3,278,426 + BPHYT_RS14360 0.72 +0.6 3,278,426 + BPHYT_RS14360 0.72 -1.0 3,278,427 - BPHYT_RS14360 0.73 +0.1 3,278,427 - BPHYT_RS14360 0.73 -0.8 3,278,570 + BPHYT_RS14360 0.88 +0.8 3,278,570 + BPHYT_RS14360 0.88 -0.8 3,278,571 - BPHYT_RS14360 0.88 +1.4 3,278,682 - +1.6
Or see this region's nucleotide sequence