Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12205 and BPHYT_RS12210 are separated by 392 nucleotides BPHYT_RS12210 and BPHYT_RS12215 are separated by 11 nucleotides BPHYT_RS12215 and BPHYT_RS12220 are separated by 134 nucleotides
BPHYT_RS12205: BPHYT_RS12205 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 2,786,630 to 2,787,514
_RS12205
BPHYT_RS12210: BPHYT_RS12210 - cardiolipin synthetase, at 2,787,907 to 2,789,358
_RS12210
BPHYT_RS12215: BPHYT_RS12215 - glutathione peroxidase, at 2,789,370 to 2,789,849
_RS12215
BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936
_RS12220
Position (kb)
2787
2788
2789
2790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2787.146 kb on + strand, within BPHYT_RS12205 at 2787.147 kb on - strand, within BPHYT_RS12205 at 2787.240 kb on - strand, within BPHYT_RS12205 at 2787.296 kb on - strand, within BPHYT_RS12205 at 2788.048 kb on + strand at 2788.049 kb on - strand at 2788.049 kb on - strand at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.066 kb on + strand, within BPHYT_RS12210 at 2788.305 kb on + strand, within BPHYT_RS12210 at 2788.432 kb on + strand, within BPHYT_RS12210 at 2788.478 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.576 kb on - strand, within BPHYT_RS12210 at 2788.636 kb on + strand, within BPHYT_RS12210 at 2788.637 kb on - strand, within BPHYT_RS12210 at 2788.637 kb on - strand, within BPHYT_RS12210 at 2789.047 kb on + strand, within BPHYT_RS12210 at 2789.048 kb on - strand, within BPHYT_RS12210 at 2789.053 kb on + strand, within BPHYT_RS12210 at 2789.054 kb on - strand, within BPHYT_RS12210 at 2789.372 kb on - strand at 2789.425 kb on + strand, within BPHYT_RS12215 at 2789.425 kb on + strand, within BPHYT_RS12215 at 2789.695 kb on + strand, within BPHYT_RS12215 at 2789.695 kb on + strand, within BPHYT_RS12215 at 2789.696 kb on - strand, within BPHYT_RS12215 at 2789.696 kb on - strand, within BPHYT_RS12215 at 2789.704 kb on + strand, within BPHYT_RS12215 at 2789.704 kb on + strand, within BPHYT_RS12215 at 2789.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,787,146 + BPHYT_RS12205 0.58 +2.3 2,787,147 - BPHYT_RS12205 0.58 +0.5 2,787,240 - BPHYT_RS12205 0.69 +0.7 2,787,296 - BPHYT_RS12205 0.75 +0.7 2,788,048 + -1.0 2,788,049 - -0.8 2,788,049 - +1.6 2,788,066 + BPHYT_RS12210 0.11 +0.7 2,788,066 + BPHYT_RS12210 0.11 +0.3 2,788,066 + BPHYT_RS12210 0.11 -0.7 2,788,305 + BPHYT_RS12210 0.27 +2.3 2,788,432 + BPHYT_RS12210 0.36 -1.4 2,788,478 - BPHYT_RS12210 0.39 -0.3 2,788,576 - BPHYT_RS12210 0.46 -0.0 2,788,576 - BPHYT_RS12210 0.46 +0.3 2,788,576 - BPHYT_RS12210 0.46 -0.1 2,788,636 + BPHYT_RS12210 0.50 -0.3 2,788,637 - BPHYT_RS12210 0.50 -1.7 2,788,637 - BPHYT_RS12210 0.50 +1.0 2,789,047 + BPHYT_RS12210 0.79 -2.8 2,789,048 - BPHYT_RS12210 0.79 -0.2 2,789,053 + BPHYT_RS12210 0.79 -2.0 2,789,054 - BPHYT_RS12210 0.79 -0.6 2,789,372 - -0.8 2,789,425 + BPHYT_RS12215 0.11 -0.5 2,789,425 + BPHYT_RS12215 0.11 -1.3 2,789,695 + BPHYT_RS12215 0.68 +0.1 2,789,695 + BPHYT_RS12215 0.68 +1.7 2,789,696 - BPHYT_RS12215 0.68 +0.1 2,789,696 - BPHYT_RS12215 0.68 +0.5 2,789,704 + BPHYT_RS12215 0.70 -0.6 2,789,704 + BPHYT_RS12215 0.70 +0.9 2,789,786 - +0.3
Or see this region's nucleotide sequence