Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotides BPHYT_RS11540 and BPHYT_RS11545 overlap by 4 nucleotides BPHYT_RS11545 and BPHYT_RS11550 are separated by 76 nucleotides BPHYT_RS11550 and BPHYT_RS11555 overlap by 4 nucleotides
BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862
_RS11535
BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810
_RS11540
BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820
_RS11545
BPHYT_RS11550: BPHYT_RS11550 - OHCU decarboxylase, at 2,625,897 to 2,626,418
_RS11550
BPHYT_RS11555: BPHYT_RS11555 - allantoinase, at 2,626,415 to 2,627,365
_RS11555
Position (kb)
2624
2625
2626 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2623.860 kb on + strand at 2623.861 kb on - strand at 2623.985 kb on - strand at 2624.020 kb on - strand at 2624.080 kb on + strand at 2624.081 kb on - strand at 2624.127 kb on + strand at 2624.129 kb on + strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.212 kb on + strand at 2624.363 kb on + strand, within BPHYT_RS11540 at 2624.400 kb on - strand, within BPHYT_RS11540 at 2624.539 kb on + strand, within BPHYT_RS11540 at 2624.540 kb on - strand, within BPHYT_RS11540 at 2625.315 kb on + strand, within BPHYT_RS11545 at 2625.316 kb on - strand, within BPHYT_RS11545 at 2625.316 kb on - strand, within BPHYT_RS11545 at 2625.349 kb on - strand, within BPHYT_RS11545 at 2625.373 kb on - strand, within BPHYT_RS11545 at 2625.655 kb on - strand, within BPHYT_RS11545 at 2625.898 kb on + strand at 2626.623 kb on + strand, within BPHYT_RS11555
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,623,860 + -1.9 2,623,861 - +0.4 2,623,985 - -1.5 2,624,020 - -0.4 2,624,080 + -0.7 2,624,081 - +3.1 2,624,127 + -0.3 2,624,129 + -0.6 2,624,130 - -0.3 2,624,130 - -0.6 2,624,130 - +1.5 2,624,212 + +0.7 2,624,363 + BPHYT_RS11540 0.13 -0.1 2,624,400 - BPHYT_RS11540 0.20 +0.3 2,624,539 + BPHYT_RS11540 0.47 +0.1 2,624,540 - BPHYT_RS11540 0.47 +0.5 2,625,315 + BPHYT_RS11545 0.50 -0.4 2,625,316 - BPHYT_RS11545 0.50 -1.8 2,625,316 - BPHYT_RS11545 0.50 -0.2 2,625,349 - BPHYT_RS11545 0.53 -2.4 2,625,373 - BPHYT_RS11545 0.56 -0.4 2,625,655 - BPHYT_RS11545 0.84 -0.8 2,625,898 + +0.1 2,626,623 + BPHYT_RS11555 0.22 +1.7
Or see this region's nucleotide sequence