Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11545

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotidesBPHYT_RS11540 and BPHYT_RS11545 overlap by 4 nucleotidesBPHYT_RS11545 and BPHYT_RS11550 are separated by 76 nucleotidesBPHYT_RS11550 and BPHYT_RS11555 overlap by 4 nucleotides BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862 _RS11535 BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810 _RS11540 BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820 _RS11545 BPHYT_RS11550: BPHYT_RS11550 - OHCU decarboxylase, at 2,625,897 to 2,626,418 _RS11550 BPHYT_RS11555: BPHYT_RS11555 - allantoinase, at 2,626,415 to 2,627,365 _RS11555 Position (kb) 2624 2625 2626Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2623.860 kb on + strandat 2623.861 kb on - strandat 2623.985 kb on - strandat 2624.020 kb on - strandat 2624.080 kb on + strandat 2624.081 kb on - strandat 2624.127 kb on + strandat 2624.129 kb on + strandat 2624.130 kb on - strandat 2624.130 kb on - strandat 2624.130 kb on - strandat 2624.212 kb on + strandat 2624.363 kb on + strand, within BPHYT_RS11540at 2624.400 kb on - strand, within BPHYT_RS11540at 2624.539 kb on + strand, within BPHYT_RS11540at 2624.540 kb on - strand, within BPHYT_RS11540at 2625.315 kb on + strand, within BPHYT_RS11545at 2625.316 kb on - strand, within BPHYT_RS11545at 2625.316 kb on - strand, within BPHYT_RS11545at 2625.349 kb on - strand, within BPHYT_RS11545at 2625.373 kb on - strand, within BPHYT_RS11545at 2625.655 kb on - strand, within BPHYT_RS11545at 2625.898 kb on + strandat 2626.623 kb on + strand, within BPHYT_RS11555

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,623,860 + -1.9
2,623,861 - +0.4
2,623,985 - -1.5
2,624,020 - -0.4
2,624,080 + -0.7
2,624,081 - +3.1
2,624,127 + -0.3
2,624,129 + -0.6
2,624,130 - -0.3
2,624,130 - -0.6
2,624,130 - +1.5
2,624,212 + +0.7
2,624,363 + BPHYT_RS11540 0.13 -0.1
2,624,400 - BPHYT_RS11540 0.20 +0.3
2,624,539 + BPHYT_RS11540 0.47 +0.1
2,624,540 - BPHYT_RS11540 0.47 +0.5
2,625,315 + BPHYT_RS11545 0.50 -0.4
2,625,316 - BPHYT_RS11545 0.50 -1.8
2,625,316 - BPHYT_RS11545 0.50 -0.2
2,625,349 - BPHYT_RS11545 0.53 -2.4
2,625,373 - BPHYT_RS11545 0.56 -0.4
2,625,655 - BPHYT_RS11545 0.84 -0.8
2,625,898 + +0.1
2,626,623 + BPHYT_RS11555 0.22 +1.7

Or see this region's nucleotide sequence