Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10190 and BPHYT_RS10195 are separated by 76 nucleotides BPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotides BPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotides
BPHYT_RS10190: BPHYT_RS10190 - phosphonate monoester hydrolase, at 2,319,705 to 2,321,276
_RS10190
BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690
_RS10195
BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561
_RS10200
BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344
_RS10205
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2320.377 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.398 kb on + strand, within BPHYT_RS10190 at 2320.428 kb on + strand, within BPHYT_RS10190 at 2320.755 kb on + strand, within BPHYT_RS10190 at 2320.941 kb on + strand, within BPHYT_RS10190 at 2320.942 kb on - strand, within BPHYT_RS10190 at 2321.237 kb on + strand at 2321.437 kb on + strand at 2321.438 kb on - strand at 2321.467 kb on - strand at 2321.467 kb on - strand at 2322.023 kb on + strand, within BPHYT_RS10195 at 2322.023 kb on + strand, within BPHYT_RS10195 at 2322.412 kb on + strand, within BPHYT_RS10195 at 2322.451 kb on + strand, within BPHYT_RS10195 at 2322.568 kb on + strand at 2322.597 kb on - strand at 2322.617 kb on + strand at 2322.857 kb on - strand at 2322.930 kb on - strand at 2322.954 kb on - strand at 2323.118 kb on + strand at 2323.119 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,320,377 + BPHYT_RS10190 0.43 -3.8 2,320,398 + BPHYT_RS10190 0.44 -0.5 2,320,398 + BPHYT_RS10190 0.44 -0.8 2,320,428 + BPHYT_RS10190 0.46 -1.1 2,320,755 + BPHYT_RS10190 0.67 -1.2 2,320,941 + BPHYT_RS10190 0.79 +0.9 2,320,942 - BPHYT_RS10190 0.79 -1.3 2,321,237 + +1.4 2,321,437 + +0.5 2,321,438 - -1.9 2,321,467 - +0.1 2,321,467 - -0.4 2,322,023 + BPHYT_RS10195 0.50 -0.6 2,322,023 + BPHYT_RS10195 0.50 -0.7 2,322,412 + BPHYT_RS10195 0.79 -0.2 2,322,451 + BPHYT_RS10195 0.82 -0.4 2,322,568 + -1.0 2,322,597 - -0.4 2,322,617 + +0.9 2,322,857 - +1.1 2,322,930 - -0.5 2,322,954 - -0.1 2,323,118 + -0.2 2,323,119 - +0.7
Or see this region's nucleotide sequence