Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09465

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09460 and BPHYT_RS09465 are separated by 341 nucleotidesBPHYT_RS09465 and BPHYT_RS09470 are separated by 83 nucleotidesBPHYT_RS09470 and BPHYT_RS09475 are separated by 43 nucleotidesBPHYT_RS09475 and BPHYT_RS09480 are separated by 86 nucleotides BPHYT_RS09460: BPHYT_RS09460 - peptidase, at 2,144,306 to 2,146,729 _RS09460 BPHYT_RS09465: BPHYT_RS09465 - transcriptional regulator, at 2,147,071 to 2,147,343 _RS09465 BPHYT_RS09470: BPHYT_RS09470 - tRNA-Val, at 2,147,427 to 2,147,502 _RS09470 BPHYT_RS09475: BPHYT_RS09475 - tRNA-Asp, at 2,147,546 to 2,147,622 _RS09475 BPHYT_RS09480: BPHYT_RS09480 - peptidyl-prolyl cis-trans isomerase, at 2,147,709 to 2,149,646 _RS09480 Position (kb) 2147 2148Strain fitness (log2 ratio) -1 0 1at 2146.840 kb on + strandat 2146.877 kb on + strandat 2146.877 kb on + strandat 2146.878 kb on - strandat 2146.878 kb on - strandat 2146.878 kb on - strandat 2146.878 kb on - strandat 2146.909 kb on + strandat 2146.910 kb on - strandat 2146.910 kb on - strandat 2146.910 kb on - strandat 2146.978 kb on + strandat 2147.172 kb on + strand, within BPHYT_RS09465at 2147.172 kb on + strand, within BPHYT_RS09465at 2147.173 kb on - strand, within BPHYT_RS09465at 2147.624 kb on + strandat 2147.674 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,146,840 + +0.8
2,146,877 + -0.1
2,146,877 + -1.0
2,146,878 - +0.4
2,146,878 - +0.3
2,146,878 - -0.8
2,146,878 - -1.1
2,146,909 + +1.7
2,146,910 - +0.7
2,146,910 - -0.3
2,146,910 - +0.3
2,146,978 + -0.4
2,147,172 + BPHYT_RS09465 0.37 -0.9
2,147,172 + BPHYT_RS09465 0.37 -1.4
2,147,173 - BPHYT_RS09465 0.37 -0.5
2,147,624 + +0.4
2,147,674 - +1.3

Or see this region's nucleotide sequence