Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09460 and BPHYT_RS09465 are separated by 341 nucleotides BPHYT_RS09465 and BPHYT_RS09470 are separated by 83 nucleotides BPHYT_RS09470 and BPHYT_RS09475 are separated by 43 nucleotides BPHYT_RS09475 and BPHYT_RS09480 are separated by 86 nucleotides
BPHYT_RS09460: BPHYT_RS09460 - peptidase, at 2,144,306 to 2,146,729
_RS09460
BPHYT_RS09465: BPHYT_RS09465 - transcriptional regulator, at 2,147,071 to 2,147,343
_RS09465
BPHYT_RS09470: BPHYT_RS09470 - tRNA-Val, at 2,147,427 to 2,147,502
_RS09470
BPHYT_RS09475: BPHYT_RS09475 - tRNA-Asp, at 2,147,546 to 2,147,622
_RS09475
BPHYT_RS09480: BPHYT_RS09480 - peptidyl-prolyl cis-trans isomerase, at 2,147,709 to 2,149,646
_RS09480
Position (kb)
2147
2148 Strain fitness (log2 ratio)
-1
0
1 at 2146.840 kb on + strand at 2146.877 kb on + strand at 2146.877 kb on + strand at 2146.878 kb on - strand at 2146.878 kb on - strand at 2146.878 kb on - strand at 2146.878 kb on - strand at 2146.909 kb on + strand at 2146.910 kb on - strand at 2146.910 kb on - strand at 2146.910 kb on - strand at 2146.978 kb on + strand at 2147.172 kb on + strand, within BPHYT_RS09465 at 2147.172 kb on + strand, within BPHYT_RS09465 at 2147.173 kb on - strand, within BPHYT_RS09465 at 2147.624 kb on + strand at 2147.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,146,840 + +0.8 2,146,877 + -0.1 2,146,877 + -1.0 2,146,878 - +0.4 2,146,878 - +0.3 2,146,878 - -0.8 2,146,878 - -1.1 2,146,909 + +1.7 2,146,910 - +0.7 2,146,910 - -0.3 2,146,910 - +0.3 2,146,978 + -0.4 2,147,172 + BPHYT_RS09465 0.37 -0.9 2,147,172 + BPHYT_RS09465 0.37 -1.4 2,147,173 - BPHYT_RS09465 0.37 -0.5 2,147,624 + +0.4 2,147,674 - +1.3
Or see this region's nucleotide sequence