Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09015 and BPHYT_RS09020 are separated by 2 nucleotides BPHYT_RS09020 and BPHYT_RS09025 are separated by 294 nucleotides BPHYT_RS09025 and BPHYT_RS09030 are separated by 235 nucleotides
BPHYT_RS09015: BPHYT_RS09015 - multidrug transporter MatE, at 2,039,518 to 2,040,897
_RS09015
BPHYT_RS09020: BPHYT_RS09020 - UDP phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase, at 2,040,900 to 2,042,657
_RS09020
BPHYT_RS09025: BPHYT_RS09025 - 50S ribosomal protein L31 type B, at 2,042,952 to 2,043,218
_RS09025
BPHYT_RS09030: BPHYT_RS09030 - membrane protein, at 2,043,454 to 2,044,290
_RS09030
Position (kb)
2040
2041
2042
2043 Strain fitness (log2 ratio)
-1
0
1 at 2040.056 kb on + strand, within BPHYT_RS09015 at 2040.056 kb on + strand, within BPHYT_RS09015 at 2040.143 kb on + strand, within BPHYT_RS09015 at 2040.165 kb on - strand, within BPHYT_RS09015 at 2040.470 kb on + strand, within BPHYT_RS09015 at 2040.666 kb on - strand, within BPHYT_RS09015 at 2040.693 kb on + strand, within BPHYT_RS09015 at 2040.734 kb on + strand, within BPHYT_RS09015 at 2040.746 kb on + strand, within BPHYT_RS09015 at 2040.746 kb on + strand, within BPHYT_RS09015 at 2040.747 kb on - strand, within BPHYT_RS09015 at 2041.058 kb on - strand at 2043.391 kb on - strand at 2043.477 kb on + strand at 2043.477 kb on + strand at 2043.507 kb on - strand at 2043.524 kb on + strand at 2043.569 kb on - strand, within BPHYT_RS09030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,040,056 + BPHYT_RS09015 0.39 -1.2 2,040,056 + BPHYT_RS09015 0.39 -0.2 2,040,143 + BPHYT_RS09015 0.45 -0.2 2,040,165 - BPHYT_RS09015 0.47 -0.7 2,040,470 + BPHYT_RS09015 0.69 -0.7 2,040,666 - BPHYT_RS09015 0.83 +0.2 2,040,693 + BPHYT_RS09015 0.85 -0.0 2,040,734 + BPHYT_RS09015 0.88 -0.7 2,040,746 + BPHYT_RS09015 0.89 +0.7 2,040,746 + BPHYT_RS09015 0.89 -0.3 2,040,747 - BPHYT_RS09015 0.89 +0.1 2,041,058 - -0.1 2,043,391 - +0.2 2,043,477 + -0.3 2,043,477 + -1.0 2,043,507 - -0.5 2,043,524 + +1.3 2,043,569 - BPHYT_RS09030 0.14 -0.9
Or see this region's nucleotide sequence