Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08835 and BPHYT_RS08840 are separated by 306 nucleotides BPHYT_RS08840 and BPHYT_RS08845 are separated by 145 nucleotides BPHYT_RS08845 and BPHYT_RS08850 overlap by 11 nucleotides
BPHYT_RS08835: BPHYT_RS08835 - glyoxylate carboligase, at 2,001,768 to 2,003,543
_RS08835
BPHYT_RS08840: BPHYT_RS08840 - LysR family transcriptional regulator, at 2,003,850 to 2,004,764
_RS08840
BPHYT_RS08845: BPHYT_RS08845 - RNA polymerase sigma factor, at 2,004,910 to 2,005,644
_RS08845
BPHYT_RS08850: BPHYT_RS08850 - transcriptional regulator, at 2,005,634 to 2,006,569
_RS08850
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2002.941 kb on - strand, within BPHYT_RS08835 at 2002.994 kb on - strand, within BPHYT_RS08835 at 2003.011 kb on + strand, within BPHYT_RS08835 at 2003.215 kb on + strand, within BPHYT_RS08835 at 2003.256 kb on - strand, within BPHYT_RS08835 at 2003.256 kb on - strand, within BPHYT_RS08835 at 2003.281 kb on + strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.327 kb on + strand, within BPHYT_RS08835 at 2003.363 kb on - strand, within BPHYT_RS08835 at 2003.363 kb on - strand, within BPHYT_RS08835 at 2003.579 kb on - strand at 2003.808 kb on + strand at 2003.808 kb on + strand at 2004.360 kb on + strand, within BPHYT_RS08840 at 2004.456 kb on + strand, within BPHYT_RS08840 at 2004.457 kb on - strand, within BPHYT_RS08840 at 2004.646 kb on - strand, within BPHYT_RS08840 at 2004.700 kb on - strand at 2004.700 kb on - strand at 2004.726 kb on + strand at 2005.013 kb on - strand, within BPHYT_RS08845 at 2005.159 kb on + strand, within BPHYT_RS08845 at 2005.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,002,941 - BPHYT_RS08835 0.66 -0.2 2,002,994 - BPHYT_RS08835 0.69 +0.0 2,003,011 + BPHYT_RS08835 0.70 +3.6 2,003,215 + BPHYT_RS08835 0.81 -0.7 2,003,256 - BPHYT_RS08835 0.84 +0.7 2,003,256 - BPHYT_RS08835 0.84 +1.0 2,003,281 + BPHYT_RS08835 0.85 -0.7 2,003,282 - BPHYT_RS08835 0.85 +1.3 2,003,282 - BPHYT_RS08835 0.85 -1.2 2,003,282 - BPHYT_RS08835 0.85 -0.1 2,003,327 + BPHYT_RS08835 0.88 +0.9 2,003,363 - BPHYT_RS08835 0.90 -0.9 2,003,363 - BPHYT_RS08835 0.90 -0.2 2,003,579 - +0.5 2,003,808 + +0.6 2,003,808 + -0.1 2,004,360 + BPHYT_RS08840 0.56 -2.0 2,004,456 + BPHYT_RS08840 0.66 -1.1 2,004,457 - BPHYT_RS08840 0.66 -0.5 2,004,646 - BPHYT_RS08840 0.87 +0.5 2,004,700 - +0.3 2,004,700 - +2.3 2,004,726 + -0.2 2,005,013 - BPHYT_RS08845 0.14 +0.6 2,005,159 + BPHYT_RS08845 0.34 +0.2 2,005,642 + -1.3
Or see this region's nucleotide sequence