Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07855 and BPHYT_RS07860 overlap by 4 nucleotides BPHYT_RS07860 and BPHYT_RS07865 are separated by 38 nucleotides BPHYT_RS07865 and BPHYT_RS07870 overlap by 4 nucleotides BPHYT_RS07870 and BPHYT_RS07875 are separated by 96 nucleotides
BPHYT_RS07855: BPHYT_RS07855 - benzoate 1,2-dioxygenase subunit alpha, at 1,789,628 to 1,790,986
_RS07855
BPHYT_RS07860: BPHYT_RS07860 - benzoate 1,2-dioxygenase subunit beta, at 1,790,983 to 1,791,471
_RS07860
BPHYT_RS07865: BPHYT_RS07865 - NADH oxidase, at 1,791,510 to 1,792,532
_RS07865
BPHYT_RS07870: BPHYT_RS07870 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 1,792,529 to 1,793,305
_RS07870
BPHYT_RS07875: BPHYT_RS07875 - CoA transferase, at 1,793,402 to 1,795,393
_RS07875
Position (kb) 1791
1792
1793 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1790.605 kb on - strand, within BPHYT_RS07855 at 1790.605 kb on - strand, within BPHYT_RS07855 at 1791.073 kb on + strand, within BPHYT_RS07860 at 1791.092 kb on - strand, within BPHYT_RS07860 at 1791.160 kb on + strand, within BPHYT_RS07860 at 1791.436 kb on + strand at 1791.436 kb on + strand at 1791.490 kb on - strand at 1791.490 kb on - strand at 1791.497 kb on + strand at 1792.309 kb on - strand, within BPHYT_RS07865 at 1792.887 kb on - strand, within BPHYT_RS07870 at 1792.986 kb on - strand, within BPHYT_RS07870 at 1793.259 kb on - strand at 1793.443 kb on - strand at 1793.443 kb on - strand at 1793.446 kb on + strand at 1793.449 kb on - strand at 1793.449 kb on - strand at 1793.449 kb on - strand at 1793.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,790,605 - BPHYT_RS07855 0.72 -1.6 1,790,605 - BPHYT_RS07855 0.72 -0.3 1,791,073 + BPHYT_RS07860 0.18 -1.0 1,791,092 - BPHYT_RS07860 0.22 +2.4 1,791,160 + BPHYT_RS07860 0.36 +0.5 1,791,436 + -2.3 1,791,436 + -0.9 1,791,490 - +3.1 1,791,490 - -0.4 1,791,497 + -0.5 1,792,309 - BPHYT_RS07865 0.78 +1.4 1,792,887 - BPHYT_RS07870 0.46 -0.3 1,792,986 - BPHYT_RS07870 0.59 -0.3 1,793,259 - -0.8 1,793,443 - +1.4 1,793,443 - +0.8 1,793,446 + -0.1 1,793,449 - +0.1 1,793,449 - +0.2 1,793,449 - -1.5 1,793,449 - -1.0
Or see this region's nucleotide sequence