Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04510 and BPHYT_RS04515 are separated by 58 nucleotides BPHYT_RS04515 and BPHYT_RS04520 are separated by 15 nucleotides BPHYT_RS04520 and BPHYT_RS04525 are separated by 175 nucleotides BPHYT_RS04525 and BPHYT_RS04530 are separated by 251 nucleotides
BPHYT_RS04510: BPHYT_RS04510 - urease subunit alpha, at 1,041,700 to 1,043,406
_RS04510
BPHYT_RS04515: BPHYT_RS04515 - urease subunit beta, at 1,043,465 to 1,043,770
_RS04515
BPHYT_RS04520: BPHYT_RS04520 - urease subunit gamma, at 1,043,786 to 1,044,088
_RS04520
BPHYT_RS04525: BPHYT_RS04525 - amino acid ABC transporter substrate-binding protein, at 1,044,264 to 1,045,067
_RS04525
BPHYT_RS04530: BPHYT_RS04530 - acyltransferase, at 1,045,319 to 1,046,404
_RS04530
Position (kb)
1044
1045
1046 Strain fitness (log2 ratio)
-2
-1
0
1 at 1044.155 kb on + strand at 1044.682 kb on + strand, within BPHYT_RS04525 at 1044.683 kb on - strand, within BPHYT_RS04525 at 1044.685 kb on + strand, within BPHYT_RS04525 at 1044.969 kb on - strand, within BPHYT_RS04525 at 1045.147 kb on + strand at 1045.452 kb on + strand, within BPHYT_RS04530 at 1045.452 kb on + strand, within BPHYT_RS04530 at 1045.452 kb on + strand, within BPHYT_RS04530 at 1045.452 kb on + strand, within BPHYT_RS04530 at 1045.453 kb on - strand, within BPHYT_RS04530 at 1045.575 kb on + strand, within BPHYT_RS04530 at 1045.575 kb on + strand, within BPHYT_RS04530 at 1045.575 kb on + strand, within BPHYT_RS04530 at 1045.576 kb on - strand, within BPHYT_RS04530 at 1045.576 kb on - strand, within BPHYT_RS04530 at 1045.576 kb on - strand, within BPHYT_RS04530 at 1045.605 kb on - strand, within BPHYT_RS04530 at 1045.605 kb on - strand, within BPHYT_RS04530 at 1045.693 kb on - strand, within BPHYT_RS04530 at 1045.770 kb on + strand, within BPHYT_RS04530
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,044,155 + +0.4 1,044,682 + BPHYT_RS04525 0.52 -0.1 1,044,683 - BPHYT_RS04525 0.52 +0.0 1,044,685 + BPHYT_RS04525 0.52 -1.0 1,044,969 - BPHYT_RS04525 0.88 +0.1 1,045,147 + +0.7 1,045,452 + BPHYT_RS04530 0.12 +0.3 1,045,452 + BPHYT_RS04530 0.12 +0.7 1,045,452 + BPHYT_RS04530 0.12 -0.4 1,045,452 + BPHYT_RS04530 0.12 -0.9 1,045,453 - BPHYT_RS04530 0.12 -0.9 1,045,575 + BPHYT_RS04530 0.24 +0.9 1,045,575 + BPHYT_RS04530 0.24 -0.7 1,045,575 + BPHYT_RS04530 0.24 -0.8 1,045,576 - BPHYT_RS04530 0.24 +1.0 1,045,576 - BPHYT_RS04530 0.24 +0.0 1,045,576 - BPHYT_RS04530 0.24 -0.9 1,045,605 - BPHYT_RS04530 0.26 -2.4 1,045,605 - BPHYT_RS04530 0.26 -1.7 1,045,693 - BPHYT_RS04530 0.34 +0.5 1,045,770 + BPHYT_RS04530 0.42 -1.3
Or see this region's nucleotide sequence