Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04345 and BPHYT_RS04350 are separated by 125 nucleotides BPHYT_RS04350 and BPHYT_RS04355 are separated by 15 nucleotides BPHYT_RS04355 and BPHYT_RS04360 overlap by 4 nucleotides
BPHYT_RS04345: BPHYT_RS04345 - NAD-dependent epimerase/dehydratase, at 1,000,129 to 1,001,067
_RS04345
BPHYT_RS04350: BPHYT_RS04350 - membrane protein, at 1,001,193 to 1,002,908
_RS04350
BPHYT_RS04355: BPHYT_RS04355 - short-chain dehydrogenase, at 1,002,924 to 1,003,664
_RS04355
BPHYT_RS04360: BPHYT_RS04360 - FAD-linked oxidase, at 1,003,661 to 1,004,977
_RS04360
Position (kb)
1001
1002
1003 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1001.112 kb on - strand at 1001.122 kb on - strand at 1001.122 kb on - strand at 1001.122 kb on - strand at 1001.336 kb on - strand at 1001.355 kb on + strand at 1001.356 kb on - strand at 1001.451 kb on - strand, within BPHYT_RS04350 at 1001.464 kb on + strand, within BPHYT_RS04350 at 1001.465 kb on - strand, within BPHYT_RS04350 at 1001.572 kb on + strand, within BPHYT_RS04350 at 1001.684 kb on - strand, within BPHYT_RS04350 at 1001.725 kb on + strand, within BPHYT_RS04350 at 1001.726 kb on - strand, within BPHYT_RS04350 at 1001.890 kb on + strand at 1001.976 kb on + strand, within BPHYT_RS04350 at 1002.074 kb on - strand, within BPHYT_RS04350 at 1002.215 kb on - strand, within BPHYT_RS04350 at 1002.316 kb on - strand, within BPHYT_RS04350 at 1002.387 kb on - strand, within BPHYT_RS04350 at 1002.526 kb on - strand, within BPHYT_RS04350 at 1002.670 kb on + strand, within BPHYT_RS04350 at 1002.670 kb on + strand, within BPHYT_RS04350 at 1002.722 kb on + strand, within BPHYT_RS04350 at 1002.722 kb on + strand, within BPHYT_RS04350 at 1002.767 kb on + strand at 1002.769 kb on + strand at 1002.769 kb on + strand at 1002.770 kb on - strand at 1002.770 kb on - strand at 1002.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,001,112 - +0.3 1,001,122 - -0.7 1,001,122 - -0.1 1,001,122 - +0.6 1,001,336 - -4.2 1,001,355 + -3.0 1,001,356 - -3.3 1,001,451 - BPHYT_RS04350 0.15 -2.7 1,001,464 + BPHYT_RS04350 0.16 +1.4 1,001,465 - BPHYT_RS04350 0.16 -4.6 1,001,572 + BPHYT_RS04350 0.22 -0.6 1,001,684 - BPHYT_RS04350 0.29 -1.9 1,001,725 + BPHYT_RS04350 0.31 -2.6 1,001,726 - BPHYT_RS04350 0.31 -1.1 1,001,890 + +1.4 1,001,976 + BPHYT_RS04350 0.46 +0.9 1,002,074 - BPHYT_RS04350 0.51 -3.9 1,002,215 - BPHYT_RS04350 0.60 -3.4 1,002,316 - BPHYT_RS04350 0.65 -0.4 1,002,387 - BPHYT_RS04350 0.70 -1.0 1,002,526 - BPHYT_RS04350 0.78 -2.7 1,002,670 + BPHYT_RS04350 0.86 -2.7 1,002,670 + BPHYT_RS04350 0.86 +0.4 1,002,722 + BPHYT_RS04350 0.89 -1.1 1,002,722 + BPHYT_RS04350 0.89 -2.8 1,002,767 + +3.4 1,002,769 + -0.4 1,002,769 + -3.5 1,002,770 - -0.9 1,002,770 - -0.7 1,002,770 - +0.4
Or see this region's nucleotide sequence