Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02355 and BPHYT_RS02360 are separated by 155 nucleotides BPHYT_RS02360 and BPHYT_RS02365 are separated by 71 nucleotides BPHYT_RS02365 and BPHYT_RS02370 are separated by 53 nucleotides BPHYT_RS02370 and BPHYT_RS02375 overlap by 4 nucleotides
BPHYT_RS02355: BPHYT_RS02355 - ferritin, at 540,161 to 541,033
_RS02355
BPHYT_RS02360: BPHYT_RS02360 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 541,189 to 541,671
_RS02360
BPHYT_RS02365: BPHYT_RS02365 - hypothetical protein, at 541,743 to 541,982
_RS02365
BPHYT_RS02370: BPHYT_RS02370 - type III pantothenate kinase, at 542,036 to 542,848
_RS02370
BPHYT_RS02375: BPHYT_RS02375 - biotin--protein ligase, at 542,845 to 543,738
_RS02375
Position (kb) 541
542 Strain fitness (log2 ratio) -1
0
1 at 541.151 kb on + strand at 541.151 kb on + strand at 541.151 kb on + strand at 541.659 kb on + strand at 541.659 kb on + strand at 541.721 kb on - strand at 541.721 kb on - strand at 541.744 kb on + strand at 541.745 kb on - strand at 541.944 kb on - strand, within BPHYT_RS02365 at 541.959 kb on + strand at 541.960 kb on - strand at 541.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 541,151 + +0.3 541,151 + -1.1 541,151 + -0.2 541,659 + -1.1 541,659 + +0.4 541,721 - +0.1 541,721 - -0.1 541,744 + +1.1 541,745 - -0.3 541,944 - BPHYT_RS02365 0.84 -1.3 541,959 + -0.3 541,960 - +0.6 541,960 - +0.0
Or see this region's nucleotide sequence