Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35250

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotidesBPHYT_RS35240 and BPHYT_RS35245 are separated by 128 nucleotidesBPHYT_RS35245 and BPHYT_RS35250 are separated by 55 nucleotidesBPHYT_RS35250 and BPHYT_RS35255 are separated by 149 nucleotides BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320 _RS35235 BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956 _RS35240 BPHYT_RS35245: BPHYT_RS35245 - hypothetical protein, at 3,510,085 to 3,510,261 _RS35245 BPHYT_RS35250: BPHYT_RS35250 - alcohol dehydrogenase, at 3,510,317 to 3,511,282 _RS35250 BPHYT_RS35255: BPHYT_RS35255 - AraC family transcriptional regulator, at 3,511,432 to 3,512,262 _RS35255 Position (kb) 3510 3511 3512Strain fitness (log2 ratio) -1 0 1at 3509.325 kb on + strandat 3509.628 kb on + strand, within BPHYT_RS35240at 3509.628 kb on + strand, within BPHYT_RS35240at 3509.628 kb on + strand, within BPHYT_RS35240at 3509.628 kb on + strand, within BPHYT_RS35240at 3509.730 kb on + strand, within BPHYT_RS35240at 3509.784 kb on + strand, within BPHYT_RS35240at 3509.785 kb on - strand, within BPHYT_RS35240at 3509.944 kb on - strandat 3510.087 kb on - strandat 3510.129 kb on + strand, within BPHYT_RS35245at 3510.129 kb on + strand, within BPHYT_RS35245at 3510.210 kb on - strand, within BPHYT_RS35245at 3510.270 kb on + strandat 3510.717 kb on + strand, within BPHYT_RS35250at 3511.869 kb on - strand, within BPHYT_RS35255at 3511.883 kb on + strand, within BPHYT_RS35255at 3511.995 kb on + strand, within BPHYT_RS35255at 3512.181 kb on + strandat 3512.199 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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3,509,325 + -1.5
3,509,628 + BPHYT_RS35240 0.43 +0.2
3,509,628 + BPHYT_RS35240 0.43 +0.5
3,509,628 + BPHYT_RS35240 0.43 +0.1
3,509,628 + BPHYT_RS35240 0.43 -1.6
3,509,730 + BPHYT_RS35240 0.61 -0.1
3,509,784 + BPHYT_RS35240 0.70 -0.2
3,509,785 - BPHYT_RS35240 0.70 -0.5
3,509,944 - -1.7
3,510,087 - +1.2
3,510,129 + BPHYT_RS35245 0.25 -0.1
3,510,129 + BPHYT_RS35245 0.25 +0.3
3,510,210 - BPHYT_RS35245 0.71 +0.1
3,510,270 + -0.1
3,510,717 + BPHYT_RS35250 0.41 -1.0
3,511,869 - BPHYT_RS35255 0.53 -0.4
3,511,883 + BPHYT_RS35255 0.54 -0.9
3,511,995 + BPHYT_RS35255 0.68 +0.6
3,512,181 + -0.5
3,512,199 - -0.3

Or see this region's nucleotide sequence