Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35090

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35085 and BPHYT_RS35090 are separated by 117 nucleotidesBPHYT_RS35090 and BPHYT_RS35095 are separated by 24 nucleotides BPHYT_RS35085: BPHYT_RS35085 - transcriptional regulator, at 3,472,890 to 3,473,819 _RS35085 BPHYT_RS35090: BPHYT_RS35090 - diaminopimelate decarboxylase, at 3,473,937 to 3,475,178 _RS35090 BPHYT_RS35095: BPHYT_RS35095 - LysR family transcriptional regulator, at 3,475,203 to 3,476,123 _RS35095 Position (kb) 3473 3474 3475 3476Strain fitness (log2 ratio) -1 0 1 2at 3473.215 kb on + strand, within BPHYT_RS35085at 3473.573 kb on + strand, within BPHYT_RS35085at 3473.901 kb on + strandat 3474.001 kb on - strandat 3474.366 kb on - strand, within BPHYT_RS35090at 3474.505 kb on - strand, within BPHYT_RS35090at 3474.991 kb on + strand, within BPHYT_RS35090at 3475.065 kb on + strandat 3475.269 kb on - strandat 3475.334 kb on + strand, within BPHYT_RS35095at 3475.520 kb on + strand, within BPHYT_RS35095

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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3,473,215 + BPHYT_RS35085 0.35 -0.6
3,473,573 + BPHYT_RS35085 0.73 +0.8
3,473,901 + +1.1
3,474,001 - -0.8
3,474,366 - BPHYT_RS35090 0.35 -1.7
3,474,505 - BPHYT_RS35090 0.46 -0.1
3,474,991 + BPHYT_RS35090 0.85 -1.2
3,475,065 + -0.5
3,475,269 - +2.0
3,475,334 + BPHYT_RS35095 0.14 +1.6
3,475,520 + BPHYT_RS35095 0.34 +1.3

Or see this region's nucleotide sequence