Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotides BPHYT_RS31025 and BPHYT_RS31030 are separated by 114 nucleotides BPHYT_RS31030 and BPHYT_RS31035 are separated by 57 nucleotides BPHYT_RS31035 and BPHYT_RS31040 are separated by 2 nucleotides
BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555
_RS31020
BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412
_RS31025
BPHYT_RS31030: BPHYT_RS31030 - tyrosine protein kinase, at 2,515,527 to 2,517,752
_RS31030
BPHYT_RS31035: BPHYT_RS31035 - protein-tyrosine-phosphatase, at 2,517,810 to 2,518,247
_RS31035
BPHYT_RS31040: BPHYT_RS31040 - sugar transporter, at 2,518,250 to 2,519,407
_RS31040
Position (kb) 2515
2516
2517
2518 Strain fitness (log2 ratio) -1
0
1 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.571 kb on - strand at 2516.262 kb on + strand, within BPHYT_RS31030 at 2516.263 kb on - strand, within BPHYT_RS31030 at 2516.556 kb on - strand, within BPHYT_RS31030 at 2516.945 kb on - strand, within BPHYT_RS31030 at 2517.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,515,298 - BPHYT_RS31025 0.87 +1.7 2,515,298 - BPHYT_RS31025 0.87 -0.9 2,515,571 - +0.8 2,516,262 + BPHYT_RS31030 0.33 +0.9 2,516,263 - BPHYT_RS31030 0.33 -0.6 2,516,556 - BPHYT_RS31030 0.46 +0.4 2,516,945 - BPHYT_RS31030 0.64 +0.0 2,517,729 - -0.4
Or see this region's nucleotide sequence