Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31010 and BPHYT_RS31015 overlap by 4 nucleotides BPHYT_RS31015 and BPHYT_RS31020 overlap by 17 nucleotides BPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotides BPHYT_RS31025 and BPHYT_RS31030 are separated by 114 nucleotides
BPHYT_RS31010: BPHYT_RS31010 - arabinose 5-phosphate isomerase, at 2,512,294 to 2,513,229
_RS31010
BPHYT_RS31015: BPHYT_RS31015 - 3-deoxy-manno-octulosonate cytidylyltransferase 2, at 2,513,226 to 2,514,017
_RS31015
BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555
_RS31020
BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412
_RS31025
BPHYT_RS31030: BPHYT_RS31030 - tyrosine protein kinase, at 2,515,527 to 2,517,752
_RS31030
Position (kb)
2514
2515 Strain fitness (log2 ratio)
-1
0
1 at 2513.214 kb on - strand at 2513.440 kb on + strand, within BPHYT_RS31015 at 2513.441 kb on - strand, within BPHYT_RS31015 at 2514.048 kb on - strand at 2514.237 kb on - strand, within BPHYT_RS31020 at 2515.298 kb on - strand, within BPHYT_RS31025 at 2515.298 kb on - strand, within BPHYT_RS31025
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,513,214 - +1.6 2,513,440 + BPHYT_RS31015 0.27 -0.7 2,513,441 - BPHYT_RS31015 0.27 +0.5 2,514,048 - -0.5 2,514,237 - BPHYT_RS31020 0.43 -1.0 2,515,298 - BPHYT_RS31025 0.87 +1.7 2,515,298 - BPHYT_RS31025 0.87 -0.9
Or see this region's nucleotide sequence