Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30325

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30320 and BPHYT_RS30325 are separated by 99 nucleotidesBPHYT_RS30325 and BPHYT_RS30330 overlap by 4 nucleotidesBPHYT_RS30330 and BPHYT_RS30335 are separated by 11 nucleotides BPHYT_RS30320: BPHYT_RS30320 - MFS transporter, at 2,357,667 to 2,358,989 _RS30320 BPHYT_RS30325: BPHYT_RS30325 - 4-oxalomesaconate hydratase, at 2,359,089 to 2,360,138 _RS30325 BPHYT_RS30330: BPHYT_RS30330 - hypothetical protein, at 2,360,135 to 2,360,803 _RS30330 BPHYT_RS30335: BPHYT_RS30335 - hypothetical protein, at 2,360,815 to 2,361,213 _RS30335 Position (kb) 2359 2360 2361Strain fitness (log2 ratio) -1 0 1 2at 2358.100 kb on - strand, within BPHYT_RS30320at 2358.163 kb on - strand, within BPHYT_RS30320at 2358.438 kb on - strand, within BPHYT_RS30320at 2358.705 kb on + strand, within BPHYT_RS30320at 2359.114 kb on - strandat 2359.411 kb on + strand, within BPHYT_RS30325at 2359.566 kb on + strand, within BPHYT_RS30325at 2359.576 kb on - strand, within BPHYT_RS30325at 2359.576 kb on - strand, within BPHYT_RS30325at 2359.662 kb on + strand, within BPHYT_RS30325at 2359.829 kb on - strand, within BPHYT_RS30325at 2359.980 kb on + strand, within BPHYT_RS30325at 2359.981 kb on - strand, within BPHYT_RS30325at 2360.558 kb on + strand, within BPHYT_RS30330at 2360.611 kb on - strand, within BPHYT_RS30330at 2361.031 kb on + strand, within BPHYT_RS30335at 2361.032 kb on - strand, within BPHYT_RS30335

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,358,100 - BPHYT_RS30320 0.33 -0.4
2,358,163 - BPHYT_RS30320 0.37 -0.4
2,358,438 - BPHYT_RS30320 0.58 -0.1
2,358,705 + BPHYT_RS30320 0.78 +0.0
2,359,114 - -0.3
2,359,411 + BPHYT_RS30325 0.31 -0.3
2,359,566 + BPHYT_RS30325 0.45 +0.2
2,359,576 - BPHYT_RS30325 0.46 +0.1
2,359,576 - BPHYT_RS30325 0.46 -0.2
2,359,662 + BPHYT_RS30325 0.55 -0.1
2,359,829 - BPHYT_RS30325 0.70 -1.2
2,359,980 + BPHYT_RS30325 0.85 -1.1
2,359,981 - BPHYT_RS30325 0.85 -1.0
2,360,558 + BPHYT_RS30330 0.63 +2.4
2,360,611 - BPHYT_RS30330 0.71 +0.9
2,361,031 + BPHYT_RS30335 0.54 -0.1
2,361,032 - BPHYT_RS30335 0.54 -1.1

Or see this region's nucleotide sequence