Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27645 and BPHYT_RS27650 are separated by 58 nucleotides BPHYT_RS27650 and BPHYT_RS27655 are separated by 436 nucleotides BPHYT_RS27655 and BPHYT_RS27660 are separated by 110 nucleotides BPHYT_RS27660 and BPHYT_RS27665 are separated by 22 nucleotides
BPHYT_RS27645: BPHYT_RS27645 - universal stress protein UspA, at 1,770,518 to 1,770,976
_RS27645
BPHYT_RS27650: BPHYT_RS27650 - hypothetical protein, at 1,771,035 to 1,771,382
_RS27650
BPHYT_RS27655: BPHYT_RS27655 - potassium transporter, at 1,771,819 to 1,772,652
_RS27655
BPHYT_RS27660: BPHYT_RS27660 - hypothetical protein, at 1,772,763 to 1,773,203
_RS27660
BPHYT_RS27665: BPHYT_RS27665 - flavodoxin, at 1,773,226 to 1,773,738
_RS27665
Position (kb)
1771
1772
1773 Strain fitness (log2 ratio)
-1
0
1 at 1770.981 kb on - strand at 1771.459 kb on + strand at 1772.330 kb on - strand, within BPHYT_RS27655 at 1772.468 kb on + strand, within BPHYT_RS27655 at 1772.833 kb on + strand, within BPHYT_RS27660 at 1772.833 kb on + strand, within BPHYT_RS27660 at 1772.864 kb on - strand, within BPHYT_RS27660 at 1773.461 kb on + strand, within BPHYT_RS27665 at 1773.524 kb on + strand, within BPHYT_RS27665
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,770,981 - +0.8 1,771,459 + +0.6 1,772,330 - BPHYT_RS27655 0.61 -0.5 1,772,468 + BPHYT_RS27655 0.78 -1.3 1,772,833 + BPHYT_RS27660 0.16 -1.3 1,772,833 + BPHYT_RS27660 0.16 -0.9 1,772,864 - BPHYT_RS27660 0.23 -1.3 1,773,461 + BPHYT_RS27665 0.46 +0.6 1,773,524 + BPHYT_RS27665 0.58 +0.6
Or see this region's nucleotide sequence