Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27615 and BPHYT_RS27620 are separated by 432 nucleotides BPHYT_RS27620 and BPHYT_RS27625 are separated by 72 nucleotides BPHYT_RS27625 and BPHYT_RS27635 are separated by 604 nucleotides
BPHYT_RS27615: BPHYT_RS27615 - F0F1 ATP synthase subunit beta, at 1,764,375 to 1,765,853
_RS27615
BPHYT_RS27620: BPHYT_RS27620 - UDP-glucose 4-epimerase, at 1,766,286 to 1,766,504
_RS27620
BPHYT_RS27625: BPHYT_RS27625 - transport-associated protein, at 1,766,577 to 1,767,227
_RS27625
BPHYT_RS27635: BPHYT_RS27635 - alcohol dehydrogenase, at 1,767,832 to 1,768,872
_RS27635
Position (kb) 1766
1767
1768 Strain fitness (log2 ratio) -1
0
1 at 1766.060 kb on + strand at 1766.111 kb on + strand at 1766.411 kb on + strand, within BPHYT_RS27620 at 1766.563 kb on + strand at 1766.712 kb on + strand, within BPHYT_RS27625 at 1766.714 kb on + strand, within BPHYT_RS27625 at 1767.098 kb on - strand, within BPHYT_RS27625 at 1767.249 kb on + strand at 1767.381 kb on - strand at 1767.592 kb on + strand at 1768.015 kb on - strand, within BPHYT_RS27635 at 1768.073 kb on - strand, within BPHYT_RS27635
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,766,060 + +1.0 1,766,111 + -0.0 1,766,411 + BPHYT_RS27620 0.57 +0.8 1,766,563 + -0.0 1,766,712 + BPHYT_RS27625 0.21 +0.2 1,766,714 + BPHYT_RS27625 0.21 -0.1 1,767,098 - BPHYT_RS27625 0.80 +0.9 1,767,249 + -0.7 1,767,381 - -0.9 1,767,592 + +0.2 1,768,015 - BPHYT_RS27635 0.18 -0.8 1,768,073 - BPHYT_RS27635 0.23 +0.1
Or see this region's nucleotide sequence