Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25100

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25095 and BPHYT_RS25100 are separated by 300 nucleotidesBPHYT_RS25100 and BPHYT_RS25105 are separated by 111 nucleotides BPHYT_RS25095: BPHYT_RS25095 - AraC family transcriptional regulator, at 1,202,184 to 1,203,266 _RS25095 BPHYT_RS25100: BPHYT_RS25100 - serine dehydratase, at 1,203,567 to 1,204,961 _RS25100 BPHYT_RS25105: BPHYT_RS25105 - sarcosine oxidase subunit beta, at 1,205,073 to 1,206,317 _RS25105 Position (kb) 1203 1204 1205Strain fitness (log2 ratio) -1 0 1 2at 1202.730 kb on - strand, within BPHYT_RS25095at 1202.842 kb on + strand, within BPHYT_RS25095at 1203.524 kb on - strandat 1204.020 kb on + strand, within BPHYT_RS25100at 1204.020 kb on + strand, within BPHYT_RS25100at 1204.572 kb on + strand, within BPHYT_RS25100at 1204.573 kb on - strand, within BPHYT_RS25100at 1204.999 kb on + strandat 1205.220 kb on + strand, within BPHYT_RS25105at 1205.398 kb on - strand, within BPHYT_RS25105at 1205.398 kb on - strand, within BPHYT_RS25105at 1205.914 kb on - strand, within BPHYT_RS25105at 1205.915 kb on + strand, within BPHYT_RS25105

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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1,202,730 - BPHYT_RS25095 0.50 +2.1
1,202,842 + BPHYT_RS25095 0.61 +1.5
1,203,524 - +1.6
1,204,020 + BPHYT_RS25100 0.32 -0.1
1,204,020 + BPHYT_RS25100 0.32 +0.3
1,204,572 + BPHYT_RS25100 0.72 +0.1
1,204,573 - BPHYT_RS25100 0.72 -0.6
1,204,999 + +1.5
1,205,220 + BPHYT_RS25105 0.12 -0.9
1,205,398 - BPHYT_RS25105 0.26 -0.6
1,205,398 - BPHYT_RS25105 0.26 +0.2
1,205,914 - BPHYT_RS25105 0.68 -1.0
1,205,915 + BPHYT_RS25105 0.68 -0.1

Or see this region's nucleotide sequence