Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24745

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24735 and BPHYT_RS24740 overlap by 4 nucleotidesBPHYT_RS24740 and BPHYT_RS24745 are separated by 148 nucleotidesBPHYT_RS24745 and BPHYT_RS24750 are separated by 280 nucleotidesBPHYT_RS24750 and BPHYT_RS24755 are separated by 111 nucleotides BPHYT_RS24735: BPHYT_RS24735 - transcriptional regulator, at 1,115,779 to 1,116,606 _RS24735 BPHYT_RS24740: BPHYT_RS24740 - RNA polymerase subunit sigma-24, at 1,116,603 to 1,117,121 _RS24740 BPHYT_RS24745: BPHYT_RS24745 - transcriptional regulator, at 1,117,270 to 1,117,992 _RS24745 BPHYT_RS24750: BPHYT_RS24750 - hypothetical protein, at 1,118,273 to 1,118,542 _RS24750 BPHYT_RS24755: BPHYT_RS24755 - catalase, at 1,118,654 to 1,119,742 _RS24755 Position (kb) 1117 1118Strain fitness (log2 ratio) -1 0 1at 1116.734 kb on - strand, within BPHYT_RS24740at 1116.734 kb on - strand, within BPHYT_RS24740at 1116.802 kb on + strand, within BPHYT_RS24740at 1117.256 kb on + strandat 1117.257 kb on - strandat 1117.257 kb on - strandat 1117.470 kb on - strand, within BPHYT_RS24745at 1117.848 kb on - strand, within BPHYT_RS24745at 1118.051 kb on + strandat 1118.402 kb on + strand, within BPHYT_RS24750at 1118.440 kb on - strand, within BPHYT_RS24750at 1118.596 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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1,116,734 - BPHYT_RS24740 0.25 +0.2
1,116,734 - BPHYT_RS24740 0.25 +0.4
1,116,802 + BPHYT_RS24740 0.38 +0.8
1,117,256 + +0.6
1,117,257 - -1.3
1,117,257 - -1.0
1,117,470 - BPHYT_RS24745 0.28 -1.2
1,117,848 - BPHYT_RS24745 0.80 -1.2
1,118,051 + +0.6
1,118,402 + BPHYT_RS24750 0.48 -0.4
1,118,440 - BPHYT_RS24750 0.62 -0.4
1,118,596 - -1.6

Or see this region's nucleotide sequence