Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20090 and BPHYT_RS20095 are separated by 249 nucleotides BPHYT_RS20095 and BPHYT_RS20100 overlap by 11 nucleotides BPHYT_RS20100 and BPHYT_RS20105 are separated by 155 nucleotides
BPHYT_RS20090: BPHYT_RS20090 - nitrate ABC transporter substrate-binding protein, at 49,893 to 50,912
_RS20090
BPHYT_RS20095: BPHYT_RS20095 - transcriptional regulator, at 51,162 to 51,839
_RS20095
BPHYT_RS20100: BPHYT_RS20100 - sensor histidine kinase, at 51,829 to 53,223
_RS20100
BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419
_RS20105
Position (kb) 51
52
53
54 Strain fitness (log2 ratio) -1
0
1
2 at 50.985 kb on + strand at 51.545 kb on - strand, within BPHYT_RS20095 at 51.891 kb on - strand at 52.046 kb on + strand, within BPHYT_RS20100 at 52.078 kb on + strand, within BPHYT_RS20100 at 52.078 kb on + strand, within BPHYT_RS20100 at 52.079 kb on - strand, within BPHYT_RS20100 at 52.079 kb on - strand, within BPHYT_RS20100 at 52.683 kb on + strand, within BPHYT_RS20100 at 52.930 kb on - strand, within BPHYT_RS20100 at 53.211 kb on + strand at 53.895 kb on + strand, within BPHYT_RS20105 at 53.896 kb on - strand, within BPHYT_RS20105 at 54.035 kb on + strand, within BPHYT_RS20105 at 54.036 kb on - strand, within BPHYT_RS20105
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 50,985 + +2.4 51,545 - BPHYT_RS20095 0.56 -0.8 51,891 - +0.4 52,046 + BPHYT_RS20100 0.16 -0.9 52,078 + BPHYT_RS20100 0.18 +1.4 52,078 + BPHYT_RS20100 0.18 -1.2 52,079 - BPHYT_RS20100 0.18 -0.3 52,079 - BPHYT_RS20100 0.18 -1.1 52,683 + BPHYT_RS20100 0.61 -0.6 52,930 - BPHYT_RS20100 0.79 -0.7 53,211 + -1.7 53,895 + BPHYT_RS20105 0.50 +0.9 53,896 - BPHYT_RS20105 0.50 +1.6 54,035 + BPHYT_RS20105 0.63 +1.5 54,036 - BPHYT_RS20105 0.63 -1.1
Or see this region's nucleotide sequence