Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17515 and BPHYT_RS17520 are separated by 7 nucleotides BPHYT_RS17520 and BPHYT_RS17525 are separated by 118 nucleotides BPHYT_RS17525 and BPHYT_RS17530 are separated by 43 nucleotides
BPHYT_RS17515: BPHYT_RS17515 - peptide chain release factor 1, at 3,967,244 to 3,968,326
_RS17515
BPHYT_RS17520: BPHYT_RS17520 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 3,968,334 to 3,969,194
_RS17520
BPHYT_RS17525: BPHYT_RS17525 - glutaredoxin, at 3,969,313 to 3,969,624
_RS17525
BPHYT_RS17530: BPHYT_RS17530 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,969,668 to 3,970,267
_RS17530
Position (kb)
3968
3969
3970 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3968.459 kb on + strand, within BPHYT_RS17520 at 3968.503 kb on - strand, within BPHYT_RS17520 at 3968.758 kb on + strand, within BPHYT_RS17520 at 3968.758 kb on + strand, within BPHYT_RS17520 at 3968.916 kb on + strand, within BPHYT_RS17520 at 3968.916 kb on + strand, within BPHYT_RS17520 at 3968.917 kb on - strand, within BPHYT_RS17520 at 3968.918 kb on + strand, within BPHYT_RS17520 at 3969.058 kb on + strand, within BPHYT_RS17520 at 3969.058 kb on + strand, within BPHYT_RS17520 at 3969.058 kb on + strand, within BPHYT_RS17520 at 3969.059 kb on - strand, within BPHYT_RS17520 at 3969.207 kb on - strand at 3969.209 kb on + strand at 3969.210 kb on - strand at 3969.252 kb on + strand at 3969.278 kb on + strand at 3969.279 kb on - strand at 3969.291 kb on + strand at 3969.536 kb on - strand, within BPHYT_RS17525 at 3969.583 kb on + strand, within BPHYT_RS17525 at 3969.584 kb on - strand, within BPHYT_RS17525 at 3969.734 kb on + strand, within BPHYT_RS17530 at 3969.817 kb on + strand, within BPHYT_RS17530 at 3969.818 kb on - strand, within BPHYT_RS17530 at 3969.818 kb on - strand, within BPHYT_RS17530 at 3970.090 kb on + strand, within BPHYT_RS17530 at 3970.090 kb on + strand, within BPHYT_RS17530 at 3970.146 kb on - strand, within BPHYT_RS17530
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 3,968,459 + BPHYT_RS17520 0.15 +1.4 3,968,503 - BPHYT_RS17520 0.20 -0.2 3,968,758 + BPHYT_RS17520 0.49 -1.3 3,968,758 + BPHYT_RS17520 0.49 +2.0 3,968,916 + BPHYT_RS17520 0.68 -0.2 3,968,916 + BPHYT_RS17520 0.68 -1.7 3,968,917 - BPHYT_RS17520 0.68 +0.4 3,968,918 + BPHYT_RS17520 0.68 -0.2 3,969,058 + BPHYT_RS17520 0.84 -2.0 3,969,058 + BPHYT_RS17520 0.84 -2.3 3,969,058 + BPHYT_RS17520 0.84 +2.4 3,969,059 - BPHYT_RS17520 0.84 -0.2 3,969,207 - +2.1 3,969,209 + +0.2 3,969,210 - +1.5 3,969,252 + -0.8 3,969,278 + +0.2 3,969,279 - +2.0 3,969,291 + -0.1 3,969,536 - BPHYT_RS17525 0.71 +0.8 3,969,583 + BPHYT_RS17525 0.87 +0.6 3,969,584 - BPHYT_RS17525 0.87 -1.3 3,969,734 + BPHYT_RS17530 0.11 +0.4 3,969,817 + BPHYT_RS17530 0.25 +0.0 3,969,818 - BPHYT_RS17530 0.25 +0.7 3,969,818 - BPHYT_RS17530 0.25 +0.2 3,970,090 + BPHYT_RS17530 0.70 -0.4 3,970,090 + BPHYT_RS17530 0.70 -0.6 3,970,146 - BPHYT_RS17530 0.80 +1.0
Or see this region's nucleotide sequence