Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17400 and BPHYT_RS17405 are separated by 30 nucleotides BPHYT_RS17405 and BPHYT_RS17410 are separated by 46 nucleotides BPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotides
BPHYT_RS17400: BPHYT_RS17400 - anthranilate phosphoribosyltransferase, at 3,942,611 to 3,943,642
_RS17400
BPHYT_RS17405: BPHYT_RS17405 - indole-3-glycerol phosphate synthase, at 3,943,673 to 3,944,458
_RS17405
BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131
_RS17410
BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061
_RS17415
Position (kb)
3944
3945
3946 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3943.517 kb on + strand, within BPHYT_RS17400 at 3943.518 kb on - strand, within BPHYT_RS17400 at 3943.518 kb on - strand, within BPHYT_RS17400 at 3943.648 kb on + strand at 3943.661 kb on - strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.672 kb on + strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.673 kb on - strand at 3943.694 kb on - strand at 3943.985 kb on + strand, within BPHYT_RS17405 at 3944.492 kb on - strand at 3944.492 kb on - strand at 3944.618 kb on + strand, within BPHYT_RS17410 at 3944.622 kb on + strand, within BPHYT_RS17410 at 3944.623 kb on - strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.067 kb on + strand, within BPHYT_RS17410 at 3945.112 kb on - strand at 3946.059 kb on + strand at 3946.059 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 3,943,517 + BPHYT_RS17400 0.88 -0.4 3,943,518 - BPHYT_RS17400 0.88 -1.7 3,943,518 - BPHYT_RS17400 0.88 +0.2 3,943,648 + +0.3 3,943,661 - +0.0 3,943,672 + -0.2 3,943,672 + +0.1 3,943,672 + -0.2 3,943,672 + -0.6 3,943,672 + +0.8 3,943,673 - +0.5 3,943,673 - +0.1 3,943,673 - +0.3 3,943,673 - +1.6 3,943,673 - -0.3 3,943,694 - -0.2 3,943,985 + BPHYT_RS17405 0.40 -0.6 3,944,492 - +0.9 3,944,492 - -2.2 3,944,618 + BPHYT_RS17410 0.18 +0.0 3,944,622 + BPHYT_RS17410 0.19 +0.4 3,944,623 - BPHYT_RS17410 0.19 -0.3 3,945,067 + BPHYT_RS17410 0.90 +0.1 3,945,067 + BPHYT_RS17410 0.90 -1.1 3,945,112 - -0.6 3,946,059 + +0.8 3,946,059 + +2.0
Or see this region's nucleotide sequence