Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14130

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14120 and BPHYT_RS14125 are separated by 26 nucleotidesBPHYT_RS14125 and BPHYT_RS14130 are separated by 28 nucleotidesBPHYT_RS14130 and BPHYT_RS14135 are separated by 17 nucleotidesBPHYT_RS14135 and BPHYT_RS14140 overlap by 4 nucleotides BPHYT_RS14120: BPHYT_RS14120 - chemotaxis protein CheY, at 3,225,241 to 3,225,942 _RS14120 BPHYT_RS14125: BPHYT_RS14125 - transcriptional regulator, at 3,225,969 to 3,226,673 _RS14125 BPHYT_RS14130: BPHYT_RS14130 - phosphate ABC transporter ATP-binding protein, at 3,226,702 to 3,227,550 _RS14130 BPHYT_RS14135: BPHYT_RS14135 - phosphate transporter permease subunit PtsA, at 3,227,568 to 3,228,464 _RS14135 BPHYT_RS14140: BPHYT_RS14140 - phosphate transporter permease subunit PstC, at 3,228,461 to 3,229,462 _RS14140 Position (kb) 3226 3227 3228Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3225.714 kb on + strand, within BPHYT_RS14120at 3225.845 kb on + strand, within BPHYT_RS14120at 3225.846 kb on - strand, within BPHYT_RS14120at 3225.971 kb on - strandat 3226.036 kb on + strandat 3226.147 kb on + strand, within BPHYT_RS14125at 3226.148 kb on - strand, within BPHYT_RS14125at 3226.679 kb on - strandat 3226.679 kb on - strandat 3226.688 kb on + strandat 3226.688 kb on + strandat 3226.688 kb on + strandat 3226.688 kb on + strandat 3226.689 kb on - strandat 3226.724 kb on + strandat 3226.724 kb on + strandat 3226.799 kb on + strand, within BPHYT_RS14130at 3226.799 kb on + strand, within BPHYT_RS14130at 3226.803 kb on + strand, within BPHYT_RS14130at 3226.803 kb on + strand, within BPHYT_RS14130at 3226.803 kb on + strand, within BPHYT_RS14130at 3226.803 kb on + strand, within BPHYT_RS14130at 3226.803 kb on + strand, within BPHYT_RS14130at 3226.805 kb on + strand, within BPHYT_RS14130at 3226.805 kb on + strand, within BPHYT_RS14130at 3226.814 kb on + strand, within BPHYT_RS14130at 3226.868 kb on + strand, within BPHYT_RS14130at 3227.297 kb on + strand, within BPHYT_RS14130at 3227.297 kb on + strand, within BPHYT_RS14130at 3227.297 kb on + strand, within BPHYT_RS14130at 3227.297 kb on + strand, within BPHYT_RS14130at 3227.408 kb on + strand, within BPHYT_RS14130at 3227.544 kb on + strandat 3227.569 kb on + strandat 3227.570 kb on - strandat 3227.570 kb on - strandat 3227.689 kb on + strand, within BPHYT_RS14135at 3227.689 kb on + strand, within BPHYT_RS14135at 3228.300 kb on - strand, within BPHYT_RS14135at 3228.444 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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3,225,714 + BPHYT_RS14120 0.67 -0.4
3,225,845 + BPHYT_RS14120 0.86 -0.1
3,225,846 - BPHYT_RS14120 0.86 -0.5
3,225,971 - -0.3
3,226,036 + +0.5
3,226,147 + BPHYT_RS14125 0.25 -0.7
3,226,148 - BPHYT_RS14125 0.25 -0.2
3,226,679 - +1.1
3,226,679 - +0.9
3,226,688 + -0.1
3,226,688 + -0.4
3,226,688 + +1.3
3,226,688 + -0.0
3,226,689 - +1.4
3,226,724 + -2.1
3,226,724 + +1.7
3,226,799 + BPHYT_RS14130 0.11 -0.7
3,226,799 + BPHYT_RS14130 0.11 -0.2
3,226,803 + BPHYT_RS14130 0.12 -0.8
3,226,803 + BPHYT_RS14130 0.12 +0.0
3,226,803 + BPHYT_RS14130 0.12 +1.5
3,226,803 + BPHYT_RS14130 0.12 +1.0
3,226,803 + BPHYT_RS14130 0.12 +0.1
3,226,805 + BPHYT_RS14130 0.12 +0.5
3,226,805 + BPHYT_RS14130 0.12 +0.1
3,226,814 + BPHYT_RS14130 0.13 +1.8
3,226,868 + BPHYT_RS14130 0.20 +2.4
3,227,297 + BPHYT_RS14130 0.70 -2.0
3,227,297 + BPHYT_RS14130 0.70 +0.6
3,227,297 + BPHYT_RS14130 0.70 +3.4
3,227,297 + BPHYT_RS14130 0.70 +4.5
3,227,408 + BPHYT_RS14130 0.83 +1.1
3,227,544 + +2.4
3,227,569 + -1.6
3,227,570 - +2.2
3,227,570 - +0.8
3,227,689 + BPHYT_RS14135 0.13 +1.3
3,227,689 + BPHYT_RS14135 0.13 +0.6
3,228,300 - BPHYT_RS14135 0.82 +1.7
3,228,444 - +1.1

Or see this region's nucleotide sequence