Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10935 and BPHYT_RS10940 are separated by 269 nucleotides BPHYT_RS10940 and BPHYT_RS10945 are separated by 40 nucleotides BPHYT_RS10945 and BPHYT_RS10950 are separated by 123 nucleotides
BPHYT_RS10935: BPHYT_RS10935 - 2-keto-3-deoxygluconate permease, at 2,488,673 to 2,489,698
_RS10935
BPHYT_RS10940: BPHYT_RS10940 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, at 2,489,968 to 2,490,804
_RS10940
BPHYT_RS10945: BPHYT_RS10945 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,490,845 to 2,491,615
_RS10945
BPHYT_RS10950: BPHYT_RS10950 - transcriptional regulator, at 2,491,739 to 2,492,611
_RS10950
Position (kb)
2489
2490
2491 Strain fitness (log2 ratio)
-2
-1
0
1 at 2489.323 kb on - strand, within BPHYT_RS10935 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.091 kb on - strand, within BPHYT_RS10940 at 2490.581 kb on - strand, within BPHYT_RS10940 at 2490.586 kb on + strand, within BPHYT_RS10940 at 2490.725 kb on - strand at 2490.735 kb on - strand at 2491.325 kb on + strand, within BPHYT_RS10945 at 2491.422 kb on - strand, within BPHYT_RS10945 at 2491.579 kb on + strand at 2491.580 kb on - strand at 2491.580 kb on - strand at 2491.683 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,489,323 - BPHYT_RS10935 0.63 -1.2 2,490,082 + BPHYT_RS10940 0.14 -0.2 2,490,082 + BPHYT_RS10940 0.14 -1.6 2,490,083 - BPHYT_RS10940 0.14 +0.4 2,490,083 - BPHYT_RS10940 0.14 -2.0 2,490,083 - BPHYT_RS10940 0.14 -2.2 2,490,091 - BPHYT_RS10940 0.15 +0.0 2,490,581 - BPHYT_RS10940 0.73 +0.7 2,490,586 + BPHYT_RS10940 0.74 -0.8 2,490,725 - +1.4 2,490,735 - -0.9 2,491,325 + BPHYT_RS10945 0.62 -0.5 2,491,422 - BPHYT_RS10945 0.75 -0.6 2,491,579 + -1.1 2,491,580 - -0.9 2,491,580 - -0.2 2,491,683 + -0.0
Or see this region's nucleotide sequence