Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10940

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10935 and BPHYT_RS10940 are separated by 269 nucleotidesBPHYT_RS10940 and BPHYT_RS10945 are separated by 40 nucleotidesBPHYT_RS10945 and BPHYT_RS10950 are separated by 123 nucleotides BPHYT_RS10935: BPHYT_RS10935 - 2-keto-3-deoxygluconate permease, at 2,488,673 to 2,489,698 _RS10935 BPHYT_RS10940: BPHYT_RS10940 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, at 2,489,968 to 2,490,804 _RS10940 BPHYT_RS10945: BPHYT_RS10945 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,490,845 to 2,491,615 _RS10945 BPHYT_RS10950: BPHYT_RS10950 - transcriptional regulator, at 2,491,739 to 2,492,611 _RS10950 Position (kb) 2489 2490 2491Strain fitness (log2 ratio) -2 -1 0 1at 2489.323 kb on - strand, within BPHYT_RS10935at 2490.082 kb on + strand, within BPHYT_RS10940at 2490.082 kb on + strand, within BPHYT_RS10940at 2490.083 kb on - strand, within BPHYT_RS10940at 2490.083 kb on - strand, within BPHYT_RS10940at 2490.083 kb on - strand, within BPHYT_RS10940at 2490.091 kb on - strand, within BPHYT_RS10940at 2490.581 kb on - strand, within BPHYT_RS10940at 2490.586 kb on + strand, within BPHYT_RS10940at 2490.725 kb on - strandat 2490.735 kb on - strandat 2491.325 kb on + strand, within BPHYT_RS10945at 2491.422 kb on - strand, within BPHYT_RS10945at 2491.579 kb on + strandat 2491.580 kb on - strandat 2491.580 kb on - strandat 2491.683 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,489,323 - BPHYT_RS10935 0.63 -1.2
2,490,082 + BPHYT_RS10940 0.14 -0.2
2,490,082 + BPHYT_RS10940 0.14 -1.6
2,490,083 - BPHYT_RS10940 0.14 +0.4
2,490,083 - BPHYT_RS10940 0.14 -2.0
2,490,083 - BPHYT_RS10940 0.14 -2.2
2,490,091 - BPHYT_RS10940 0.15 +0.0
2,490,581 - BPHYT_RS10940 0.73 +0.7
2,490,586 + BPHYT_RS10940 0.74 -0.8
2,490,725 - +1.4
2,490,735 - -0.9
2,491,325 + BPHYT_RS10945 0.62 -0.5
2,491,422 - BPHYT_RS10945 0.75 -0.6
2,491,579 + -1.1
2,491,580 - -0.9
2,491,580 - -0.2
2,491,683 + -0.0

Or see this region's nucleotide sequence