Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10925 and BPHYT_RS10930 are separated by 86 nucleotides BPHYT_RS10930 and BPHYT_RS10935 are separated by 67 nucleotides BPHYT_RS10935 and BPHYT_RS10940 are separated by 269 nucleotides
BPHYT_RS10925: BPHYT_RS10925 - aldehyde dehydrogenase, at 2,486,474 to 2,487,919
_RS10925
BPHYT_RS10930: BPHYT_RS10930 - GCN5 family N-acetyltransferase, at 2,488,006 to 2,488,605
_RS10930
BPHYT_RS10935: BPHYT_RS10935 - 2-keto-3-deoxygluconate permease, at 2,488,673 to 2,489,698
_RS10935
BPHYT_RS10940: BPHYT_RS10940 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase, at 2,489,968 to 2,490,804
_RS10940
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2487.716 kb on + strand, within BPHYT_RS10925 at 2487.717 kb on - strand, within BPHYT_RS10925 at 2487.881 kb on + strand at 2487.917 kb on + strand at 2488.175 kb on - strand, within BPHYT_RS10930 at 2488.353 kb on + strand, within BPHYT_RS10930 at 2488.366 kb on + strand, within BPHYT_RS10930 at 2488.542 kb on + strand, within BPHYT_RS10930 at 2488.543 kb on - strand, within BPHYT_RS10930 at 2488.635 kb on + strand at 2488.639 kb on + strand at 2489.323 kb on - strand, within BPHYT_RS10935 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.082 kb on + strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.083 kb on - strand, within BPHYT_RS10940 at 2490.091 kb on - strand, within BPHYT_RS10940 at 2490.581 kb on - strand, within BPHYT_RS10940 at 2490.586 kb on + strand, within BPHYT_RS10940
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,487,716 + BPHYT_RS10925 0.86 -0.7 2,487,717 - BPHYT_RS10925 0.86 +0.2 2,487,881 + -0.3 2,487,917 + +2.4 2,488,175 - BPHYT_RS10930 0.28 -1.2 2,488,353 + BPHYT_RS10930 0.58 -0.3 2,488,366 + BPHYT_RS10930 0.60 -0.7 2,488,542 + BPHYT_RS10930 0.89 -0.6 2,488,543 - BPHYT_RS10930 0.90 -0.8 2,488,635 + +0.2 2,488,639 + +0.5 2,489,323 - BPHYT_RS10935 0.63 -1.2 2,490,082 + BPHYT_RS10940 0.14 -0.2 2,490,082 + BPHYT_RS10940 0.14 -1.6 2,490,083 - BPHYT_RS10940 0.14 +0.4 2,490,083 - BPHYT_RS10940 0.14 -2.0 2,490,083 - BPHYT_RS10940 0.14 -2.2 2,490,091 - BPHYT_RS10940 0.15 +0.0 2,490,581 - BPHYT_RS10940 0.73 +0.7 2,490,586 + BPHYT_RS10940 0.74 -0.8
Or see this region's nucleotide sequence