Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09965

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09960 and BPHYT_RS09965 are separated by 218 nucleotidesBPHYT_RS09965 and BPHYT_RS09970 are separated by 74 nucleotidesBPHYT_RS09970 and BPHYT_RS09975 are separated by 139 nucleotides BPHYT_RS09960: BPHYT_RS09960 - short-chain dehydrogenase, at 2,268,317 to 2,269,147 _RS09960 BPHYT_RS09965: BPHYT_RS09965 - AraC family transcriptional regulator, at 2,269,366 to 2,270,313 _RS09965 BPHYT_RS09970: BPHYT_RS09970 - glutathione S-transferase, at 2,270,388 to 2,271,011 _RS09970 BPHYT_RS09975: BPHYT_RS09975 - hypothetical protein, at 2,271,151 to 2,271,495 _RS09975 Position (kb) 2269 2270 2271Strain fitness (log2 ratio) -2 -1 0 1at 2268.650 kb on + strand, within BPHYT_RS09960at 2268.650 kb on + strand, within BPHYT_RS09960at 2268.706 kb on - strand, within BPHYT_RS09960at 2268.706 kb on - strand, within BPHYT_RS09960at 2268.708 kb on + strand, within BPHYT_RS09960at 2269.516 kb on + strand, within BPHYT_RS09965at 2269.517 kb on - strand, within BPHYT_RS09965at 2269.580 kb on - strand, within BPHYT_RS09965at 2269.586 kb on - strand, within BPHYT_RS09965at 2269.600 kb on + strand, within BPHYT_RS09965at 2269.601 kb on - strand, within BPHYT_RS09965at 2269.675 kb on + strand, within BPHYT_RS09965at 2270.074 kb on - strand, within BPHYT_RS09965at 2270.089 kb on + strand, within BPHYT_RS09965at 2270.601 kb on + strand, within BPHYT_RS09970at 2270.602 kb on - strand, within BPHYT_RS09970at 2270.716 kb on - strand, within BPHYT_RS09970at 2270.802 kb on - strand, within BPHYT_RS09970at 2270.949 kb on + strandat 2270.950 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,268,650 + BPHYT_RS09960 0.40 +0.2
2,268,650 + BPHYT_RS09960 0.40 +0.6
2,268,706 - BPHYT_RS09960 0.47 -1.0
2,268,706 - BPHYT_RS09960 0.47 -0.1
2,268,708 + BPHYT_RS09960 0.47 -1.2
2,269,516 + BPHYT_RS09965 0.16 +0.1
2,269,517 - BPHYT_RS09965 0.16 -0.9
2,269,580 - BPHYT_RS09965 0.23 +0.4
2,269,586 - BPHYT_RS09965 0.23 +0.1
2,269,600 + BPHYT_RS09965 0.25 -0.6
2,269,601 - BPHYT_RS09965 0.25 -0.9
2,269,675 + BPHYT_RS09965 0.33 -1.9
2,270,074 - BPHYT_RS09965 0.75 -0.5
2,270,089 + BPHYT_RS09965 0.76 -2.1
2,270,601 + BPHYT_RS09970 0.34 -0.1
2,270,602 - BPHYT_RS09970 0.34 +0.9
2,270,716 - BPHYT_RS09970 0.53 -1.4
2,270,802 - BPHYT_RS09970 0.66 -0.2
2,270,949 + -0.8
2,270,950 - -2.3

Or see this region's nucleotide sequence