Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02920 and BPHYT_RS02925 overlap by 4 nucleotides BPHYT_RS02925 and BPHYT_RS02930 overlap by 1 nucleotides BPHYT_RS02930 and BPHYT_RS02935 are separated by 161 nucleotides
BPHYT_RS02920: BPHYT_RS02920 - hypothetical protein, at 649,565 to 650,173
_RS02920
BPHYT_RS02925: BPHYT_RS02925 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 650,170 to 650,730
_RS02925
BPHYT_RS02930: BPHYT_RS02930 - KpsF/GutQ family protein, at 650,730 to 651,713
_RS02930
BPHYT_RS02935: BPHYT_RS02935 - potassium transporter, at 651,875 to 653,878
_RS02935
Position (kb) 650
651
652 Strain fitness (log2 ratio) -2
-1
0
1 at 651.107 kb on - strand, within BPHYT_RS02930 at 651.107 kb on - strand, within BPHYT_RS02930 at 651.723 kb on - strand at 651.724 kb on + strand at 651.724 kb on + strand at 651.724 kb on + strand at 651.856 kb on + strand at 651.857 kb on - strand at 652.324 kb on + strand, within BPHYT_RS02935 at 652.464 kb on + strand, within BPHYT_RS02935 at 652.465 kb on - strand, within BPHYT_RS02935
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 651,107 - BPHYT_RS02930 0.38 +1.1 651,107 - BPHYT_RS02930 0.38 -1.9 651,723 - +0.4 651,724 + +1.1 651,724 + -0.9 651,724 + +0.6 651,856 + +1.0 651,857 - -1.5 652,324 + BPHYT_RS02935 0.22 -2.0 652,464 + BPHYT_RS02935 0.29 -0.6 652,465 - BPHYT_RS02935 0.29 -1.9
Or see this region's nucleotide sequence