Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00155 and BPHYT_RS00160 are separated by 124 nucleotides BPHYT_RS00160 and BPHYT_RS00165 are separated by 131 nucleotides BPHYT_RS00165 and BPHYT_RS00170 are separated by 22 nucleotides
BPHYT_RS00155: BPHYT_RS00155 - aminotransferase, at 41,982 to 43,136
_RS00155
BPHYT_RS00160: BPHYT_RS00160 - ABC transporter substrate-binding protein, at 43,261 to 44,049
_RS00160
BPHYT_RS00165: BPHYT_RS00165 - AraC family transcriptional regulator, at 44,181 to 45,026
_RS00165
BPHYT_RS00170: BPHYT_RS00170 - lysine transporter LysE, at 45,049 to 45,636
_RS00170
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1
2 at 42.291 kb on + strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.360 kb on + strand, within BPHYT_RS00155 at 42.361 kb on - strand, within BPHYT_RS00155 at 42.591 kb on + strand, within BPHYT_RS00155 at 42.739 kb on + strand, within BPHYT_RS00155 at 42.801 kb on + strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 43.044 kb on - strand at 43.052 kb on + strand at 43.140 kb on + strand at 43.141 kb on - strand at 43.173 kb on + strand at 43.173 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.456 kb on - strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 44.047 kb on + strand at 44.048 kb on - strand at 44.048 kb on - strand at 44.059 kb on + strand at 44.059 kb on + strand at 44.129 kb on + strand at 44.130 kb on - strand at 44.133 kb on + strand at 44.155 kb on + strand at 44.163 kb on + strand at 44.178 kb on + strand at 44.302 kb on - strand, within BPHYT_RS00165 at 44.362 kb on - strand, within BPHYT_RS00165 at 44.847 kb on + strand, within BPHYT_RS00165 at 44.868 kb on + strand, within BPHYT_RS00165 at 44.868 kb on + strand, within BPHYT_RS00165 at 44.893 kb on - strand, within BPHYT_RS00165 at 44.893 kb on - strand, within BPHYT_RS00165 at 45.016 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 42,291 + BPHYT_RS00155 0.27 +0.9 42,337 - BPHYT_RS00155 0.31 -0.7 42,337 - BPHYT_RS00155 0.31 -0.9 42,360 + BPHYT_RS00155 0.33 +2.0 42,361 - BPHYT_RS00155 0.33 -0.5 42,591 + BPHYT_RS00155 0.53 +0.2 42,739 + BPHYT_RS00155 0.66 +1.5 42,801 + BPHYT_RS00155 0.71 +0.3 42,838 - BPHYT_RS00155 0.74 +0.4 42,838 - BPHYT_RS00155 0.74 -1.0 42,858 + BPHYT_RS00155 0.76 -0.2 42,858 + BPHYT_RS00155 0.76 -1.2 42,858 + BPHYT_RS00155 0.76 +0.9 43,044 - -0.4 43,052 + +1.0 43,140 + -0.3 43,141 - +0.9 43,173 + -0.7 43,173 + +0.3 43,185 + -0.5 43,185 + -1.2 43,185 + -0.2 43,456 - BPHYT_RS00160 0.25 +1.7 43,768 + BPHYT_RS00160 0.64 +0.1 43,768 + BPHYT_RS00160 0.64 +0.2 44,047 + -1.7 44,048 - -0.9 44,048 - -0.8 44,059 + -0.9 44,059 + -1.8 44,129 + -0.1 44,130 - +0.5 44,133 + +0.5 44,155 + -2.4 44,163 + +0.3 44,178 + -0.6 44,302 - BPHYT_RS00165 0.14 +0.3 44,362 - BPHYT_RS00165 0.21 -0.7 44,847 + BPHYT_RS00165 0.79 -0.0 44,868 + BPHYT_RS00165 0.81 -0.1 44,868 + BPHYT_RS00165 0.81 -0.5 44,893 - BPHYT_RS00165 0.84 -0.6 44,893 - BPHYT_RS00165 0.84 -0.6 45,016 - +1.3
Or see this region's nucleotide sequence