Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30145

Experiment: 36hr PHYTO9 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30140 and BPHYT_RS30145 are separated by 110 nucleotidesBPHYT_RS30145 and BPHYT_RS30150 are separated by 328 nucleotides BPHYT_RS30140: BPHYT_RS30140 - 3-methylitaconate isomerase, at 2,313,771 to 2,314,958 _RS30140 BPHYT_RS30145: BPHYT_RS30145 - aconitate hydratase, at 2,315,069 to 2,317,666 _RS30145 BPHYT_RS30150: BPHYT_RS30150 - methylcitrate synthase, at 2,317,995 to 2,319,164 _RS30150 Position (kb) 2315 2316 2317 2318Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2314.921 kb on + strandat 2314.921 kb on + strandat 2314.922 kb on - strandat 2314.922 kb on - strandat 2314.989 kb on - strandat 2315.488 kb on - strand, within BPHYT_RS30145at 2315.844 kb on + strand, within BPHYT_RS30145at 2315.844 kb on + strand, within BPHYT_RS30145at 2315.845 kb on - strand, within BPHYT_RS30145at 2315.845 kb on - strand, within BPHYT_RS30145at 2315.922 kb on + strand, within BPHYT_RS30145at 2316.973 kb on + strand, within BPHYT_RS30145at 2316.975 kb on + strand, within BPHYT_RS30145at 2316.975 kb on + strand, within BPHYT_RS30145at 2317.493 kb on - strandat 2317.560 kb on + strandat 2317.661 kb on - strandat 2317.687 kb on - strandat 2317.736 kb on + strandat 2317.739 kb on - strandat 2317.741 kb on - strandat 2317.741 kb on - strandat 2317.770 kb on + strandat 2317.770 kb on + strandat 2317.781 kb on + strandat 2317.899 kb on - strandat 2318.045 kb on - strandat 2318.170 kb on + strand, within BPHYT_RS30150at 2318.336 kb on - strand, within BPHYT_RS30150at 2318.506 kb on + strand, within BPHYT_RS30150at 2318.510 kb on + strand, within BPHYT_RS30150

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1
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2,314,921 + +2.5
2,314,921 + -1.2
2,314,922 - +0.8
2,314,922 - -0.8
2,314,989 - +0.3
2,315,488 - BPHYT_RS30145 0.16 -0.7
2,315,844 + BPHYT_RS30145 0.30 +0.5
2,315,844 + BPHYT_RS30145 0.30 +0.1
2,315,845 - BPHYT_RS30145 0.30 +0.6
2,315,845 - BPHYT_RS30145 0.30 -0.6
2,315,922 + BPHYT_RS30145 0.33 +2.5
2,316,973 + BPHYT_RS30145 0.73 +0.5
2,316,975 + BPHYT_RS30145 0.73 +0.9
2,316,975 + BPHYT_RS30145 0.73 +2.5
2,317,493 - +0.2
2,317,560 + +1.7
2,317,661 - +1.5
2,317,687 - -1.4
2,317,736 + -0.8
2,317,739 - +0.9
2,317,741 - +0.4
2,317,741 - -2.2
2,317,770 + -1.1
2,317,770 + +1.5
2,317,781 + -0.1
2,317,899 - +3.5
2,318,045 - -0.1
2,318,170 + BPHYT_RS30150 0.15 +2.9
2,318,336 - BPHYT_RS30150 0.29 -2.3
2,318,506 + BPHYT_RS30150 0.44 -1.0
2,318,510 + BPHYT_RS30150 0.44 -0.3

Or see this region's nucleotide sequence