Experiment: 36hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25495 and BPHYT_RS25500 are separated by 158 nucleotides BPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotides BPHYT_RS25505 and BPHYT_RS25510 are separated by 16 nucleotides BPHYT_RS25510 and BPHYT_RS25515 are separated by 83 nucleotides
BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007
_RS25495
BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807
_RS25500
BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349
_RS25505
BPHYT_RS25510: BPHYT_RS25510 - hypothetical protein, at 1,298,366 to 1,298,686
_RS25510
BPHYT_RS25515: BPHYT_RS25515 - glutathione S-transferase, at 1,298,770 to 1,299,471
_RS25515
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-1
0
1 at 1297.045 kb on + strand at 1297.046 kb on - strand at 1297.054 kb on + strand at 1297.222 kb on + strand at 1297.234 kb on - strand, within BPHYT_RS25500 at 1297.509 kb on - strand, within BPHYT_RS25500 at 1297.544 kb on - strand, within BPHYT_RS25500 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1299.243 kb on - strand, within BPHYT_RS25515
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1 remove 1,297,045 + +0.1 1,297,046 - -0.8 1,297,054 + +1.2 1,297,222 + -0.3 1,297,234 - BPHYT_RS25500 0.11 -0.6 1,297,509 - BPHYT_RS25500 0.53 -0.6 1,297,544 - BPHYT_RS25500 0.59 +0.2 1,298,100 + BPHYT_RS25505 0.54 -0.5 1,298,100 + BPHYT_RS25505 0.54 -0.8 1,299,243 - BPHYT_RS25515 0.67 +0.1
Or see this region's nucleotide sequence