Experiment: 36hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20010 and BPHYT_RS20015 are separated by 61 nucleotides BPHYT_RS20015 and BPHYT_RS20020 are separated by 331 nucleotides BPHYT_RS20020 and BPHYT_RS20025 are separated by 137 nucleotides
BPHYT_RS20010: BPHYT_RS20010 - membrane protein, at 33,343 to 34,482
_RS20010
BPHYT_RS20015: BPHYT_RS20015 - MFS transporter, at 34,544 to 35,791
_RS20015
BPHYT_RS20020: BPHYT_RS20020 - MarR family transcriptional regulator, at 36,123 to 36,623
_RS20020
BPHYT_RS20025: BPHYT_RS20025 - crotonase, at 36,761 to 37,588
_RS20025
Position (kb) 34
35
36 Strain fitness (log2 ratio) -2
-1
0
1 at 33.560 kb on + strand, within BPHYT_RS20010 at 33.561 kb on - strand, within BPHYT_RS20010 at 33.998 kb on + strand, within BPHYT_RS20010 at 34.046 kb on + strand, within BPHYT_RS20010 at 34.070 kb on + strand, within BPHYT_RS20010 at 34.112 kb on + strand, within BPHYT_RS20010 at 34.370 kb on + strand at 34.371 kb on - strand at 34.389 kb on + strand at 34.881 kb on + strand, within BPHYT_RS20015 at 34.881 kb on + strand, within BPHYT_RS20015 at 34.882 kb on - strand, within BPHYT_RS20015 at 35.046 kb on + strand, within BPHYT_RS20015 at 35.070 kb on + strand, within BPHYT_RS20015 at 35.325 kb on + strand, within BPHYT_RS20015 at 36.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1 remove 33,560 + BPHYT_RS20010 0.19 +0.8 33,561 - BPHYT_RS20010 0.19 +1.3 33,998 + BPHYT_RS20010 0.57 -0.1 34,046 + BPHYT_RS20010 0.62 +0.0 34,070 + BPHYT_RS20010 0.64 -1.1 34,112 + BPHYT_RS20010 0.67 +0.8 34,370 + -1.1 34,371 - +0.7 34,389 + +0.5 34,881 + BPHYT_RS20015 0.27 -1.3 34,881 + BPHYT_RS20015 0.27 -1.0 34,882 - BPHYT_RS20015 0.27 -0.2 35,046 + BPHYT_RS20015 0.40 -1.8 35,070 + BPHYT_RS20015 0.42 -0.6 35,325 + BPHYT_RS20015 0.63 -0.0 36,093 + +0.2
Or see this region's nucleotide sequence