Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12520

Experiment: 36hr PHYTO9 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12515 and BPHYT_RS12520 are separated by 291 nucleotidesBPHYT_RS12520 and BPHYT_RS12525 are separated by 21 nucleotides BPHYT_RS12515: BPHYT_RS12515 - potassium transporter Kup, at 2,856,541 to 2,858,427 _RS12515 BPHYT_RS12520: BPHYT_RS12520 - nicotinate phosphoribosyltransferase, at 2,858,719 to 2,859,291 _RS12520 BPHYT_RS12525: BPHYT_RS12525 - adenylosuccinate synthetase, at 2,859,313 to 2,860,659 _RS12525 Position (kb) 2858 2859 2860Strain fitness (log2 ratio) -1 0 1 2at 2858.537 kb on + strandat 2858.537 kb on + strandat 2858.538 kb on - strandat 2858.538 kb on - strandat 2858.667 kb on - strandat 2858.853 kb on - strand, within BPHYT_RS12520at 2859.029 kb on + strand, within BPHYT_RS12520at 2859.030 kb on - strand, within BPHYT_RS12520at 2859.030 kb on - strand, within BPHYT_RS12520at 2859.295 kb on + strandat 2859.296 kb on - strandat 2859.314 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1
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2,858,537 + -0.3
2,858,537 + -0.7
2,858,538 - -0.2
2,858,538 - -0.6
2,858,667 - -0.3
2,858,853 - BPHYT_RS12520 0.23 +2.3
2,859,029 + BPHYT_RS12520 0.54 -0.2
2,859,030 - BPHYT_RS12520 0.54 -0.3
2,859,030 - BPHYT_RS12520 0.54 +0.1
2,859,295 + -0.3
2,859,296 - -0.6
2,859,314 + -0.3

Or see this region's nucleotide sequence