Experiment: 36hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotides BPHYT_RS11540 and BPHYT_RS11545 overlap by 4 nucleotides BPHYT_RS11545 and BPHYT_RS11550 are separated by 76 nucleotides BPHYT_RS11550 and BPHYT_RS11555 overlap by 4 nucleotides
BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862
_RS11535
BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810
_RS11540
BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820
_RS11545
BPHYT_RS11550: BPHYT_RS11550 - OHCU decarboxylase, at 2,625,897 to 2,626,418
_RS11550
BPHYT_RS11555: BPHYT_RS11555 - allantoinase, at 2,626,415 to 2,627,365
_RS11555
Position (kb)
2624
2625
2626 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2623.860 kb on + strand at 2623.861 kb on - strand at 2623.985 kb on - strand at 2624.020 kb on - strand at 2624.080 kb on + strand at 2624.081 kb on - strand at 2624.127 kb on + strand at 2624.129 kb on + strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.212 kb on + strand at 2624.363 kb on + strand, within BPHYT_RS11540 at 2624.400 kb on - strand, within BPHYT_RS11540 at 2624.539 kb on + strand, within BPHYT_RS11540 at 2624.540 kb on - strand, within BPHYT_RS11540 at 2625.315 kb on + strand, within BPHYT_RS11545 at 2625.316 kb on - strand, within BPHYT_RS11545 at 2625.316 kb on - strand, within BPHYT_RS11545 at 2625.349 kb on - strand, within BPHYT_RS11545 at 2625.373 kb on - strand, within BPHYT_RS11545 at 2625.655 kb on - strand, within BPHYT_RS11545 at 2625.898 kb on + strand at 2626.623 kb on + strand, within BPHYT_RS11555
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1 remove 2,623,860 + -0.3 2,623,861 - +1.9 2,623,985 - -0.5 2,624,020 - -0.9 2,624,080 + -1.6 2,624,081 - +1.8 2,624,127 + -2.3 2,624,129 + -1.1 2,624,130 - +1.1 2,624,130 - -2.2 2,624,130 - +0.5 2,624,212 + +0.8 2,624,363 + BPHYT_RS11540 0.13 +0.8 2,624,400 - BPHYT_RS11540 0.20 -0.6 2,624,539 + BPHYT_RS11540 0.47 +0.5 2,624,540 - BPHYT_RS11540 0.47 -1.5 2,625,315 + BPHYT_RS11545 0.50 -0.7 2,625,316 - BPHYT_RS11545 0.50 +0.2 2,625,316 - BPHYT_RS11545 0.50 -0.1 2,625,349 - BPHYT_RS11545 0.53 -2.3 2,625,373 - BPHYT_RS11545 0.56 -1.5 2,625,655 - BPHYT_RS11545 0.84 -0.0 2,625,898 + +1.5 2,626,623 + BPHYT_RS11555 0.22 +0.8
Or see this region's nucleotide sequence