Experiment: 36hr PHYTO9 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10000 and BPHYT_RS10005 are separated by 200 nucleotides BPHYT_RS10005 and BPHYT_RS10010 are separated by 113 nucleotides BPHYT_RS10010 and BPHYT_RS10015 overlap by 4 nucleotides
BPHYT_RS10000: BPHYT_RS10000 - glucoamylase, at 2,277,862 to 2,279,733
_RS10000
BPHYT_RS10005: BPHYT_RS10005 - hypothetical protein, at 2,279,934 to 2,280,305
_RS10005
BPHYT_RS10010: BPHYT_RS10010 - cupin, at 2,280,419 to 2,280,850
_RS10010
BPHYT_RS10015: BPHYT_RS10015 - NAD(P)H dehydrogenase (quinone), at 2,280,847 to 2,281,530
_RS10015
Position (kb)
2279
2280
2281 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2278.966 kb on + strand, within BPHYT_RS10000 at 2279.061 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.504 kb on + strand, within BPHYT_RS10000 at 2279.505 kb on - strand, within BPHYT_RS10000 at 2279.505 kb on - strand, within BPHYT_RS10000 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.111 kb on + strand, within BPHYT_RS10005 at 2280.363 kb on - strand at 2280.369 kb on - strand at 2281.059 kb on + strand, within BPHYT_RS10015 at 2281.060 kb on - strand, within BPHYT_RS10015 at 2281.079 kb on + strand, within BPHYT_RS10015 at 2281.179 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015 at 2281.245 kb on - strand, within BPHYT_RS10015
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1 remove 2,278,966 + BPHYT_RS10000 0.59 +0.1 2,279,061 + BPHYT_RS10000 0.64 +0.9 2,279,504 + BPHYT_RS10000 0.88 -0.5 2,279,504 + BPHYT_RS10000 0.88 +0.2 2,279,504 + BPHYT_RS10000 0.88 -0.9 2,279,505 - BPHYT_RS10000 0.88 +0.3 2,279,505 - BPHYT_RS10000 0.88 +0.3 2,280,111 + BPHYT_RS10005 0.48 +0.1 2,280,111 + BPHYT_RS10005 0.48 -2.0 2,280,363 - -0.5 2,280,369 - +0.4 2,281,059 + BPHYT_RS10015 0.31 -0.6 2,281,060 - BPHYT_RS10015 0.31 -0.2 2,281,079 + BPHYT_RS10015 0.34 +1.9 2,281,179 - BPHYT_RS10015 0.49 +0.1 2,281,245 - BPHYT_RS10015 0.58 +1.9 2,281,245 - BPHYT_RS10015 0.58 +0.3 2,281,245 - BPHYT_RS10015 0.58 +0.1
Or see this region's nucleotide sequence