Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09860

Experiment: 36hr PHYTO9 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09855 and BPHYT_RS09860 are separated by 119 nucleotidesBPHYT_RS09860 and BPHYT_RS09865 are separated by 60 nucleotidesBPHYT_RS09865 and BPHYT_RS09870 are separated by 143 nucleotides BPHYT_RS09855: BPHYT_RS09855 - major facilitator superfamily protein, at 2,242,187 to 2,243,554 _RS09855 BPHYT_RS09860: BPHYT_RS09860 - pilus assembly protein PilP, at 2,243,674 to 2,244,348 _RS09860 BPHYT_RS09865: BPHYT_RS09865 - methyltransferase, at 2,244,409 to 2,244,897 _RS09865 BPHYT_RS09870: BPHYT_RS09870 - membrane protein, at 2,245,041 to 2,246,198 _RS09870 Position (kb) 2243 2244 2245Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2243.552 kb on + strandat 2243.553 kb on - strandat 2244.110 kb on - strand, within BPHYT_RS09860at 2245.115 kb on + strandat 2245.115 kb on + strandat 2245.166 kb on - strand, within BPHYT_RS09870at 2245.283 kb on - strand, within BPHYT_RS09870

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 0.1
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2,243,552 + +2.4
2,243,553 - +4.0
2,244,110 - BPHYT_RS09860 0.65 -1.9
2,245,115 + +0.4
2,245,115 + -0.4
2,245,166 - BPHYT_RS09870 0.11 +0.4
2,245,283 - BPHYT_RS09870 0.21 +0.2

Or see this region's nucleotide sequence