Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35970 and BPHYT_RS35975 are separated by 66 nucleotides BPHYT_RS35975 and BPHYT_RS35980 are separated by 165 nucleotides BPHYT_RS35980 and BPHYT_RS35985 are separated by 100 nucleotides
BPHYT_RS35970: BPHYT_RS35970 - DNA recombinase, at 27,468 to 28,574
_RS35970
BPHYT_RS35975: BPHYT_RS35975 - Xre family transcriptional regulator, at 28,641 to 28,880
_RS35975
BPHYT_RS35980: BPHYT_RS35980 - hypothetical protein, at 29,046 to 30,128
_RS35980
BPHYT_RS35985: BPHYT_RS35985 - hypothetical protein, at 30,229 to 30,975
_RS35985
Position (kb) 29
30
31 Strain fitness (log2 ratio) -2
-1
0
1
2 at 28.316 kb on + strand, within BPHYT_RS35970 at 28.418 kb on + strand, within BPHYT_RS35970 at 28.441 kb on - strand, within BPHYT_RS35970 at 28.441 kb on - strand, within BPHYT_RS35970 at 28.446 kb on - strand, within BPHYT_RS35970 at 28.553 kb on - strand at 28.579 kb on - strand at 28.770 kb on - strand, within BPHYT_RS35975 at 28.771 kb on + strand, within BPHYT_RS35975 at 28.771 kb on + strand, within BPHYT_RS35975 at 28.771 kb on + strand, within BPHYT_RS35975 at 28.771 kb on + strand, within BPHYT_RS35975 at 28.771 kb on + strand, within BPHYT_RS35975 at 28.772 kb on - strand, within BPHYT_RS35975 at 28.772 kb on - strand, within BPHYT_RS35975 at 28.772 kb on - strand, within BPHYT_RS35975 at 28.827 kb on - strand, within BPHYT_RS35975 at 28.834 kb on - strand, within BPHYT_RS35975 at 28.891 kb on + strand at 28.892 kb on - strand at 28.895 kb on + strand at 28.927 kb on - strand at 28.927 kb on - strand at 28.942 kb on - strand at 28.957 kb on + strand at 28.957 kb on + strand at 29.045 kb on - strand at 29.111 kb on + strand at 29.111 kb on + strand at 29.111 kb on + strand at 29.112 kb on - strand at 29.112 kb on - strand at 29.112 kb on - strand at 29.114 kb on - strand at 29.114 kb on - strand at 29.513 kb on - strand, within BPHYT_RS35980 at 29.599 kb on + strand, within BPHYT_RS35980 at 29.600 kb on - strand, within BPHYT_RS35980 at 29.656 kb on + strand, within BPHYT_RS35980 at 29.656 kb on + strand, within BPHYT_RS35980 at 29.657 kb on - strand, within BPHYT_RS35980 at 29.657 kb on - strand, within BPHYT_RS35980 at 29.673 kb on - strand, within BPHYT_RS35980 at 30.794 kb on + strand, within BPHYT_RS35985 at 30.795 kb on - strand, within BPHYT_RS35985 at 30.803 kb on + strand, within BPHYT_RS35985 at 30.803 kb on + strand, within BPHYT_RS35985 at 31.108 kb on + strand at 31.108 kb on + strand at 31.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 28,316 + BPHYT_RS35970 0.77 +0.2 28,418 + BPHYT_RS35970 0.86 -0.3 28,441 - BPHYT_RS35970 0.88 -0.0 28,441 - BPHYT_RS35970 0.88 +0.8 28,446 - BPHYT_RS35970 0.88 -0.1 28,553 - -1.2 28,579 - +0.6 28,770 - BPHYT_RS35975 0.54 +1.5 28,771 + BPHYT_RS35975 0.54 +0.2 28,771 + BPHYT_RS35975 0.54 -1.8 28,771 + BPHYT_RS35975 0.54 -1.7 28,771 + BPHYT_RS35975 0.54 +0.1 28,771 + BPHYT_RS35975 0.54 -1.5 28,772 - BPHYT_RS35975 0.55 -1.0 28,772 - BPHYT_RS35975 0.55 -0.2 28,772 - BPHYT_RS35975 0.55 -0.3 28,827 - BPHYT_RS35975 0.78 -0.6 28,834 - BPHYT_RS35975 0.80 +1.0 28,891 + +0.4 28,892 - +0.9 28,895 + -0.3 28,927 - -0.9 28,927 - +0.3 28,942 - -0.1 28,957 + +0.2 28,957 + -0.4 29,045 - +0.2 29,111 + +1.1 29,111 + +1.5 29,111 + +1.4 29,112 - +0.7 29,112 - +1.6 29,112 - -1.5 29,114 - -1.8 29,114 - -0.3 29,513 - BPHYT_RS35980 0.43 -0.8 29,599 + BPHYT_RS35980 0.51 +0.7 29,600 - BPHYT_RS35980 0.51 -1.6 29,656 + BPHYT_RS35980 0.56 +0.1 29,656 + BPHYT_RS35980 0.56 +1.4 29,657 - BPHYT_RS35980 0.56 +0.4 29,657 - BPHYT_RS35980 0.56 -0.3 29,673 - BPHYT_RS35980 0.58 -0.6 30,794 + BPHYT_RS35985 0.76 -1.0 30,795 - BPHYT_RS35985 0.76 -0.6 30,803 + BPHYT_RS35985 0.77 -0.6 30,803 + BPHYT_RS35985 0.77 -0.3 31,108 + -0.2 31,108 + +2.1 31,109 - +1.1
Or see this region's nucleotide sequence