Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35025 and BPHYT_RS35030 are separated by 137 nucleotides BPHYT_RS35030 and BPHYT_RS35035 are separated by 176 nucleotides BPHYT_RS35035 and BPHYT_RS35040 are separated by 351 nucleotides
BPHYT_RS35025: BPHYT_RS35025 - sarcosine oxidase, at 3,461,407 to 3,462,510
_RS35025
BPHYT_RS35030: BPHYT_RS35030 - hypothetical protein, at 3,462,648 to 3,462,851
_RS35030
BPHYT_RS35035: BPHYT_RS35035 - membrane protein, at 3,463,028 to 3,464,089
_RS35035
BPHYT_RS35040: BPHYT_RS35040 - lipocalin, at 3,464,441 to 3,465,037
_RS35040
Position (kb)
3463
3464
3465 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3462.057 kb on + strand, within BPHYT_RS35025 at 3462.270 kb on - strand, within BPHYT_RS35025 at 3462.310 kb on - strand, within BPHYT_RS35025 at 3462.356 kb on + strand, within BPHYT_RS35025 at 3462.687 kb on + strand, within BPHYT_RS35030 at 3462.716 kb on - strand, within BPHYT_RS35030 at 3462.716 kb on - strand, within BPHYT_RS35030 at 3462.763 kb on + strand, within BPHYT_RS35030 at 3462.764 kb on - strand, within BPHYT_RS35030 at 3462.820 kb on - strand, within BPHYT_RS35030 at 3462.828 kb on + strand, within BPHYT_RS35030 at 3462.829 kb on - strand, within BPHYT_RS35030 at 3462.851 kb on + strand at 3462.852 kb on - strand at 3462.852 kb on - strand at 3462.890 kb on - strand at 3463.341 kb on + strand, within BPHYT_RS35035 at 3463.341 kb on + strand, within BPHYT_RS35035 at 3463.352 kb on - strand, within BPHYT_RS35035 at 3463.518 kb on - strand, within BPHYT_RS35035 at 3463.518 kb on - strand, within BPHYT_RS35035 at 3464.128 kb on - strand at 3464.153 kb on + strand at 3464.382 kb on - strand at 3464.384 kb on + strand at 3464.384 kb on + strand at 3464.384 kb on + strand at 3464.385 kb on - strand at 3464.385 kb on - strand at 3464.436 kb on - strand at 3464.574 kb on - strand, within BPHYT_RS35040 at 3464.574 kb on - strand, within BPHYT_RS35040 at 3464.685 kb on - strand, within BPHYT_RS35040 at 3464.687 kb on + strand, within BPHYT_RS35040 at 3464.727 kb on - strand, within BPHYT_RS35040 at 3464.820 kb on - strand, within BPHYT_RS35040 at 3465.077 kb on - strand at 3465.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 3,462,057 + BPHYT_RS35025 0.59 -0.8 3,462,270 - BPHYT_RS35025 0.78 -2.3 3,462,310 - BPHYT_RS35025 0.82 -0.3 3,462,356 + BPHYT_RS35025 0.86 +0.5 3,462,687 + BPHYT_RS35030 0.19 +0.1 3,462,716 - BPHYT_RS35030 0.33 +0.2 3,462,716 - BPHYT_RS35030 0.33 +0.7 3,462,763 + BPHYT_RS35030 0.56 -0.6 3,462,764 - BPHYT_RS35030 0.57 +0.6 3,462,820 - BPHYT_RS35030 0.84 +0.2 3,462,828 + BPHYT_RS35030 0.88 -0.3 3,462,829 - BPHYT_RS35030 0.89 +0.7 3,462,851 + +0.4 3,462,852 - +0.0 3,462,852 - +0.0 3,462,890 - +0.9 3,463,341 + BPHYT_RS35035 0.29 -0.8 3,463,341 + BPHYT_RS35035 0.29 -0.3 3,463,352 - BPHYT_RS35035 0.31 -0.8 3,463,518 - BPHYT_RS35035 0.46 -0.1 3,463,518 - BPHYT_RS35035 0.46 -1.0 3,464,128 - -0.3 3,464,153 + +0.0 3,464,382 - -0.2 3,464,384 + -0.1 3,464,384 + -1.5 3,464,384 + +0.4 3,464,385 - +1.3 3,464,385 - -0.3 3,464,436 - -0.2 3,464,574 - BPHYT_RS35040 0.22 +0.7 3,464,574 - BPHYT_RS35040 0.22 +2.5 3,464,685 - BPHYT_RS35040 0.41 +1.0 3,464,687 + BPHYT_RS35040 0.41 -0.1 3,464,727 - BPHYT_RS35040 0.48 +0.6 3,464,820 - BPHYT_RS35040 0.63 +0.1 3,465,077 - +1.0 3,465,077 - -0.2
Or see this region's nucleotide sequence