Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32300

Experiment: 36hr PHYTO9 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32290 and BPHYT_RS32295 are separated by 86 nucleotidesBPHYT_RS32295 and BPHYT_RS32300 are separated by 6 nucleotidesBPHYT_RS32300 and BPHYT_RS32305 are separated by 202 nucleotidesBPHYT_RS32305 and BPHYT_RS32310 are separated by 154 nucleotides BPHYT_RS32290: BPHYT_RS32290 - hypothetical protein, at 2,814,521 to 2,815,210 _RS32290 BPHYT_RS32295: BPHYT_RS32295 - ATP-binding protein, at 2,815,297 to 2,816,085 _RS32295 BPHYT_RS32300: BPHYT_RS32300 - integrase, at 2,816,092 to 2,817,609 _RS32300 BPHYT_RS32305: BPHYT_RS32305 - hypothetical protein, at 2,817,812 to 2,818,234 _RS32305 BPHYT_RS32310: BPHYT_RS32310 - porin, at 2,818,389 to 2,819,576 _RS32310 Position (kb) 2816 2817 2818Strain fitness (log2 ratio) -1 0 1 2at 2815.094 kb on + strand, within BPHYT_RS32290at 2817.813 kb on + strandat 2817.848 kb on + strandat 2817.860 kb on + strand, within BPHYT_RS32305at 2817.948 kb on + strand, within BPHYT_RS32305at 2818.328 kb on - strandat 2818.328 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1
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2,815,094 + BPHYT_RS32290 0.83 +0.9
2,817,813 + +0.2
2,817,848 + -0.1
2,817,860 + BPHYT_RS32305 0.11 -0.7
2,817,948 + BPHYT_RS32305 0.32 +2.4
2,818,328 - +1.0
2,818,328 - -1.6

Or see this region's nucleotide sequence