Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29685 and BPHYT_RS29690 are separated by 136 nucleotides BPHYT_RS29690 and BPHYT_RS29695 are separated by 23 nucleotides BPHYT_RS29695 and BPHYT_RS29700 are separated by 162 nucleotides
BPHYT_RS29685: BPHYT_RS29685 - hypothetical protein, at 2,212,263 to 2,212,550
_RS29685
BPHYT_RS29690: BPHYT_RS29690 - exodeoxyribonuclease III, at 2,212,687 to 2,213,499
_RS29690
BPHYT_RS29695: BPHYT_RS29695 - hypothetical protein, at 2,213,523 to 2,213,741
_RS29695
BPHYT_RS29700: BPHYT_RS29700 - cytochrome C1, at 2,213,904 to 2,215,961
_RS29700
Position (kb)
2213
2214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2213.018 kb on + strand, within BPHYT_RS29690 at 2213.019 kb on - strand, within BPHYT_RS29690 at 2213.084 kb on + strand, within BPHYT_RS29690 at 2213.086 kb on + strand, within BPHYT_RS29690 at 2213.166 kb on + strand, within BPHYT_RS29690 at 2213.174 kb on + strand, within BPHYT_RS29690 at 2213.675 kb on - strand, within BPHYT_RS29695 at 2213.677 kb on + strand, within BPHYT_RS29695 at 2214.109 kb on + strand at 2214.109 kb on + strand at 2214.113 kb on - strand, within BPHYT_RS29700
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 2,213,018 + BPHYT_RS29690 0.41 -0.1 2,213,019 - BPHYT_RS29690 0.41 -0.2 2,213,084 + BPHYT_RS29690 0.49 -0.6 2,213,086 + BPHYT_RS29690 0.49 +2.5 2,213,166 + BPHYT_RS29690 0.59 -0.9 2,213,174 + BPHYT_RS29690 0.60 +0.0 2,213,675 - BPHYT_RS29695 0.69 -2.8 2,213,677 + BPHYT_RS29695 0.70 -0.1 2,214,109 + +0.0 2,214,109 + +0.8 2,214,113 - BPHYT_RS29700 0.10 -1.7
Or see this region's nucleotide sequence