Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29450

Experiment: 36hr PHYTO9 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29440 and BPHYT_RS29445 are separated by 6 nucleotidesBPHYT_RS29445 and BPHYT_RS29450 are separated by 2 nucleotidesBPHYT_RS29450 and BPHYT_RS29455 overlap by 4 nucleotidesBPHYT_RS29455 and BPHYT_RS29460 are separated by 182 nucleotides BPHYT_RS29440: BPHYT_RS29440 - LysR family transcriptional regulator, at 2,159,941 to 2,160,849 _RS29440 BPHYT_RS29445: BPHYT_RS29445 - ribose 1,5-bisphosphate phosphokinase, at 2,160,856 to 2,161,410 _RS29445 BPHYT_RS29450: BPHYT_RS29450 - phosphonate metabolism protein, at 2,161,413 to 2,162,135 _RS29450 BPHYT_RS29455: BPHYT_RS29455 - GntR family transcriptional regulator, at 2,162,132 to 2,162,890 _RS29455 BPHYT_RS29460: BPHYT_RS29460 - alkylphosphonate utilization protein, at 2,163,073 to 2,163,570 _RS29460 Position (kb) 2161 2162 2163Strain fitness (log2 ratio) -2 -1 0 1 2at 2161.296 kb on + strand, within BPHYT_RS29445at 2161.881 kb on + strand, within BPHYT_RS29450at 2162.092 kb on + strandat 2162.191 kb on - strandat 2162.982 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1
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2,161,296 + BPHYT_RS29445 0.79 +2.2
2,161,881 + BPHYT_RS29450 0.65 -2.0
2,162,092 + -0.1
2,162,191 - -0.2
2,162,982 + -0.7

Or see this region's nucleotide sequence