Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28610

Experiment: 36hr PHYTO9 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28605 and BPHYT_RS28610 are separated by 454 nucleotidesBPHYT_RS28610 and BPHYT_RS28615 are separated by 186 nucleotides BPHYT_RS28605: BPHYT_RS28605 - NADPH-dependent FMN reductase, at 1,970,036 to 1,970,758 _RS28605 BPHYT_RS28610: BPHYT_RS28610 - beta-lactamase, at 1,971,213 to 1,973,081 _RS28610 BPHYT_RS28615: BPHYT_RS28615 - 6-carboxyhexanoate--CoA ligase, at 1,973,268 to 1,975,400 _RS28615 Position (kb) 1971 1972 1973 1974Strain fitness (log2 ratio) -1 0 1 2at 1970.632 kb on - strand, within BPHYT_RS28605at 1971.157 kb on + strandat 1971.277 kb on - strandat 1971.616 kb on - strand, within BPHYT_RS28610at 1971.619 kb on + strand, within BPHYT_RS28610at 1972.477 kb on - strand, within BPHYT_RS28610at 1972.683 kb on + strand, within BPHYT_RS28610at 1973.010 kb on + strandat 1973.166 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1
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1,970,632 - BPHYT_RS28605 0.82 +2.5
1,971,157 + +1.5
1,971,277 - +0.1
1,971,616 - BPHYT_RS28610 0.22 +0.4
1,971,619 + BPHYT_RS28610 0.22 -0.5
1,972,477 - BPHYT_RS28610 0.68 -0.0
1,972,683 + BPHYT_RS28610 0.79 -0.3
1,973,010 + +0.7
1,973,166 - -0.3

Or see this region's nucleotide sequence