Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28605

Experiment: 36hr PHYTO9 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28595 and BPHYT_RS28600 are separated by 23 nucleotidesBPHYT_RS28600 and BPHYT_RS28605 are separated by 278 nucleotidesBPHYT_RS28605 and BPHYT_RS28610 are separated by 454 nucleotides BPHYT_RS28595: BPHYT_RS28595 - glyoxalase, at 1,968,763 to 1,969,239 _RS28595 BPHYT_RS28600: BPHYT_RS28600 - ArsC family transcriptional regulator, at 1,969,263 to 1,969,757 _RS28600 BPHYT_RS28605: BPHYT_RS28605 - NADPH-dependent FMN reductase, at 1,970,036 to 1,970,758 _RS28605 BPHYT_RS28610: BPHYT_RS28610 - beta-lactamase, at 1,971,213 to 1,973,081 _RS28610 Position (kb) 1970 1971Strain fitness (log2 ratio) -2 -1 0 1 2at 1969.108 kb on - strand, within BPHYT_RS28595at 1969.127 kb on + strand, within BPHYT_RS28595at 1969.276 kb on + strandat 1969.277 kb on - strandat 1969.446 kb on - strand, within BPHYT_RS28600at 1969.755 kb on + strandat 1970.023 kb on - strandat 1970.154 kb on - strand, within BPHYT_RS28605at 1970.632 kb on - strand, within BPHYT_RS28605at 1971.157 kb on + strandat 1971.277 kb on - strandat 1971.616 kb on - strand, within BPHYT_RS28610at 1971.619 kb on + strand, within BPHYT_RS28610

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1
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1,969,108 - BPHYT_RS28595 0.72 +0.2
1,969,127 + BPHYT_RS28595 0.76 +1.9
1,969,276 + +0.0
1,969,277 - +0.4
1,969,446 - BPHYT_RS28600 0.37 -2.6
1,969,755 + -0.1
1,970,023 - -0.2
1,970,154 - BPHYT_RS28605 0.16 -2.6
1,970,632 - BPHYT_RS28605 0.82 +2.5
1,971,157 + +1.5
1,971,277 - +0.1
1,971,616 - BPHYT_RS28610 0.22 +0.4
1,971,619 + BPHYT_RS28610 0.22 -0.5

Or see this region's nucleotide sequence