Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24155 and BPHYT_RS24160 are separated by 224 nucleotides BPHYT_RS24160 and BPHYT_RS24165 are separated by 51 nucleotides BPHYT_RS24165 and BPHYT_RS24170 overlap by 4 nucleotides
BPHYT_RS24155: BPHYT_RS24155 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) <small>(from data)</small>, at 987,186 to 988,103
_RS24155
BPHYT_RS24160: BPHYT_RS24160 - D-galactarate dehydratase, at 988,328 to 989,917
_RS24160
BPHYT_RS24165: BPHYT_RS24165 - NAD-dependent dehydratase, at 989,969 to 990,811
_RS24165
BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737
_RS24170
Position (kb) 988
989
990 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 987.419 kb on - strand, within BPHYT_RS24155 at 987.491 kb on - strand, within BPHYT_RS24155 at 987.491 kb on - strand, within BPHYT_RS24155 at 987.491 kb on - strand, within BPHYT_RS24155 at 987.654 kb on - strand, within BPHYT_RS24155 at 987.654 kb on - strand, within BPHYT_RS24155 at 987.760 kb on + strand, within BPHYT_RS24155 at 987.761 kb on - strand, within BPHYT_RS24155 at 987.908 kb on - strand, within BPHYT_RS24155 at 987.974 kb on - strand at 988.003 kb on + strand, within BPHYT_RS24155 at 988.003 kb on + strand, within BPHYT_RS24155 at 988.004 kb on - strand, within BPHYT_RS24155 at 988.004 kb on - strand, within BPHYT_RS24155 at 988.004 kb on - strand, within BPHYT_RS24155 at 988.007 kb on + strand, within BPHYT_RS24155 at 988.106 kb on + strand at 988.106 kb on + strand at 988.107 kb on - strand at 988.107 kb on - strand at 988.107 kb on - strand at 988.107 kb on - strand at 988.111 kb on - strand at 988.219 kb on + strand at 988.219 kb on + strand at 988.416 kb on + strand at 988.509 kb on - strand, within BPHYT_RS24160 at 988.510 kb on + strand, within BPHYT_RS24160 at 988.657 kb on + strand, within BPHYT_RS24160 at 988.657 kb on + strand, within BPHYT_RS24160 at 988.658 kb on - strand, within BPHYT_RS24160 at 988.769 kb on - strand, within BPHYT_RS24160 at 989.399 kb on + strand, within BPHYT_RS24160 at 989.409 kb on - strand, within BPHYT_RS24160 at 989.676 kb on - strand, within BPHYT_RS24160 at 989.962 kb on + strand at 989.963 kb on - strand at 989.963 kb on - strand at 989.963 kb on - strand at 990.278 kb on + strand, within BPHYT_RS24165 at 990.449 kb on + strand, within BPHYT_RS24165 at 990.452 kb on + strand, within BPHYT_RS24165 at 990.615 kb on - strand, within BPHYT_RS24165
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 987,419 - BPHYT_RS24155 0.25 +0.5 987,491 - BPHYT_RS24155 0.33 -0.5 987,491 - BPHYT_RS24155 0.33 +1.5 987,491 - BPHYT_RS24155 0.33 -1.0 987,654 - BPHYT_RS24155 0.51 +0.8 987,654 - BPHYT_RS24155 0.51 -0.3 987,760 + BPHYT_RS24155 0.63 +0.1 987,761 - BPHYT_RS24155 0.63 -1.5 987,908 - BPHYT_RS24155 0.79 -1.2 987,974 - +0.4 988,003 + BPHYT_RS24155 0.89 +0.1 988,003 + BPHYT_RS24155 0.89 -0.5 988,004 - BPHYT_RS24155 0.89 +0.5 988,004 - BPHYT_RS24155 0.89 +0.0 988,004 - BPHYT_RS24155 0.89 +0.5 988,007 + BPHYT_RS24155 0.89 +1.7 988,106 + +1.7 988,106 + -0.6 988,107 - -2.0 988,107 - -2.3 988,107 - +1.1 988,107 - +0.4 988,111 - -0.8 988,219 + -0.3 988,219 + -0.9 988,416 + -0.5 988,509 - BPHYT_RS24160 0.11 -1.4 988,510 + BPHYT_RS24160 0.11 -2.8 988,657 + BPHYT_RS24160 0.21 +0.4 988,657 + BPHYT_RS24160 0.21 +0.3 988,658 - BPHYT_RS24160 0.21 -0.3 988,769 - BPHYT_RS24160 0.28 -1.0 989,399 + BPHYT_RS24160 0.67 -0.0 989,409 - BPHYT_RS24160 0.68 +0.6 989,676 - BPHYT_RS24160 0.85 -0.1 989,962 + -0.1 989,963 - +2.4 989,963 - -0.4 989,963 - -0.7 990,278 + BPHYT_RS24165 0.37 -1.0 990,449 + BPHYT_RS24165 0.57 +0.2 990,452 + BPHYT_RS24165 0.57 -0.2 990,615 - BPHYT_RS24165 0.77 +0.1
Or see this region's nucleotide sequence