Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23260 and BPHYT_RS23265 are separated by 49 nucleotides BPHYT_RS23265 and BPHYT_RS23270 are separated by 41 nucleotides BPHYT_RS23270 and BPHYT_RS23275 are separated by 81 nucleotides
BPHYT_RS23260: BPHYT_RS23260 - isovaleryl-CoA dehydrogenase, at 782,334 to 783,515
_RS23260
BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172
_RS23265
BPHYT_RS23270: BPHYT_RS23270 - enoyl-CoA hydratase, at 785,214 to 785,999
_RS23270
BPHYT_RS23275: BPHYT_RS23275 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 786,081 to 788,159
_RS23275
Position (kb) 783
784
785
786 Strain fitness (log2 ratio) -1
0
1 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.793 kb on + strand, within BPHYT_RS23260 at 782.794 kb on - strand, within BPHYT_RS23260 at 783.142 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.331 kb on - strand, within BPHYT_RS23260 at 783.394 kb on - strand, within BPHYT_RS23260 at 783.442 kb on - strand at 783.563 kb on + strand at 783.564 kb on - strand at 783.808 kb on + strand, within BPHYT_RS23265 at 783.809 kb on - strand, within BPHYT_RS23265 at 783.913 kb on + strand, within BPHYT_RS23265 at 784.068 kb on + strand, within BPHYT_RS23265 at 784.144 kb on + strand, within BPHYT_RS23265 at 784.278 kb on + strand, within BPHYT_RS23265 at 784.433 kb on - strand, within BPHYT_RS23265 at 785.658 kb on + strand, within BPHYT_RS23270
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 782,680 - BPHYT_RS23260 0.29 -0.3 782,680 - BPHYT_RS23260 0.29 -0.9 782,793 + BPHYT_RS23260 0.39 -0.5 782,794 - BPHYT_RS23260 0.39 +0.4 783,142 - BPHYT_RS23260 0.68 +0.9 783,217 - BPHYT_RS23260 0.75 -0.1 783,217 - BPHYT_RS23260 0.75 -0.1 783,331 - BPHYT_RS23260 0.84 +0.1 783,394 - BPHYT_RS23260 0.90 +0.3 783,442 - +0.1 783,563 + -1.4 783,564 - -0.5 783,808 + BPHYT_RS23265 0.15 -1.2 783,809 - BPHYT_RS23265 0.15 +0.8 783,913 + BPHYT_RS23265 0.22 -0.9 784,068 + BPHYT_RS23265 0.31 +0.1 784,144 + BPHYT_RS23265 0.36 -1.6 784,278 + BPHYT_RS23265 0.44 -1.6 784,433 - BPHYT_RS23265 0.54 -0.4 785,658 + BPHYT_RS23270 0.56 +1.3
Or see this region's nucleotide sequence