Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotides BPHYT_RS22975 and BPHYT_RS22980 are separated by 52 nucleotides
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127
_RS22970
BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545
_RS22975
BPHYT_RS22980: BPHYT_RS22980 - LysR family transcriptional regulator, at 715,598 to 716,494
_RS22980
Position (kb) 713
714
715
716 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 712.538 kb on - strand at 713.139 kb on + strand at 713.140 kb on - strand at 713.157 kb on + strand at 713.195 kb on + strand at 713.272 kb on + strand at 713.272 kb on + strand at 713.273 kb on - strand at 713.713 kb on + strand, within BPHYT_RS22975 at 713.815 kb on + strand, within BPHYT_RS22975 at 713.846 kb on - strand, within BPHYT_RS22975 at 713.860 kb on + strand, within BPHYT_RS22975 at 714.268 kb on + strand, within BPHYT_RS22975 at 714.269 kb on - strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.479 kb on + strand, within BPHYT_RS22975 at 714.549 kb on - strand, within BPHYT_RS22975 at 715.137 kb on - strand, within BPHYT_RS22975 at 715.324 kb on - strand, within BPHYT_RS22975 at 715.534 kb on - strand at 715.539 kb on + strand at 715.674 kb on + strand at 716.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 712,538 - -0.1 713,139 + -0.3 713,140 - -0.7 713,157 + -0.1 713,195 + -1.5 713,272 + -0.5 713,272 + +0.2 713,273 - -2.5 713,713 + BPHYT_RS22975 0.15 +0.1 713,815 + BPHYT_RS22975 0.19 +0.5 713,846 - BPHYT_RS22975 0.21 +0.2 713,860 + BPHYT_RS22975 0.21 +0.0 714,268 + BPHYT_RS22975 0.40 -0.1 714,269 - BPHYT_RS22975 0.40 +0.1 714,472 + BPHYT_RS22975 0.50 +3.5 714,472 + BPHYT_RS22975 0.50 +0.1 714,479 + BPHYT_RS22975 0.50 -0.4 714,549 - BPHYT_RS22975 0.54 -1.6 715,137 - BPHYT_RS22975 0.81 +0.2 715,324 - BPHYT_RS22975 0.90 -0.4 715,534 - -0.6 715,539 + +1.1 715,674 + -0.9 716,529 + +1.0
Or see this region's nucleotide sequence