Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20645 and BPHYT_RS20650 are separated by 26 nucleotides BPHYT_RS20650 and BPHYT_RS20655 are separated by 73 nucleotides BPHYT_RS20655 and BPHYT_RS20660 are separated by 171 nucleotides
BPHYT_RS20645: BPHYT_RS20645 - RND transporter, at 192,960 to 194,378
_RS20645
BPHYT_RS20650: BPHYT_RS20650 - hypothetical protein, at 194,405 to 194,788
_RS20650
BPHYT_RS20655: BPHYT_RS20655 - hypothetical protein, at 194,862 to 195,305
_RS20655
BPHYT_RS20660: BPHYT_RS20660 - 2-methylisocitrate lyase, at 195,477 to 196,301
_RS20660
Position (kb) 194
195
196 Strain fitness (log2 ratio) -1
0
1 at 193.876 kb on - strand, within BPHYT_RS20645 at 194.176 kb on - strand, within BPHYT_RS20645 at 194.462 kb on + strand, within BPHYT_RS20650 at 194.686 kb on + strand, within BPHYT_RS20650 at 194.761 kb on + strand at 194.834 kb on + strand at 194.834 kb on + strand at 194.983 kb on + strand, within BPHYT_RS20655 at 194.984 kb on - strand, within BPHYT_RS20655 at 194.984 kb on - strand, within BPHYT_RS20655 at 194.986 kb on - strand, within BPHYT_RS20655 at 194.986 kb on - strand, within BPHYT_RS20655 at 195.154 kb on + strand, within BPHYT_RS20655 at 195.385 kb on + strand at 195.386 kb on - strand at 195.754 kb on + strand, within BPHYT_RS20660 at 195.782 kb on - strand, within BPHYT_RS20660 at 196.201 kb on + strand, within BPHYT_RS20660 at 196.202 kb on - strand, within BPHYT_RS20660
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 193,876 - BPHYT_RS20645 0.65 -1.3 194,176 - BPHYT_RS20645 0.86 -0.7 194,462 + BPHYT_RS20650 0.15 +0.3 194,686 + BPHYT_RS20650 0.73 +0.0 194,761 + +0.2 194,834 + +0.1 194,834 + +0.6 194,983 + BPHYT_RS20655 0.27 +0.0 194,984 - BPHYT_RS20655 0.27 +0.1 194,984 - BPHYT_RS20655 0.27 +0.9 194,986 - BPHYT_RS20655 0.28 -0.1 194,986 - BPHYT_RS20655 0.28 +0.4 195,154 + BPHYT_RS20655 0.66 +0.4 195,385 + -0.4 195,386 - -0.4 195,754 + BPHYT_RS20660 0.34 -0.8 195,782 - BPHYT_RS20660 0.37 -0.3 196,201 + BPHYT_RS20660 0.88 +0.1 196,202 - BPHYT_RS20660 0.88 -0.3
Or see this region's nucleotide sequence