Experiment: 36hr PHYTO9 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20445 and BPHYT_RS20450 are separated by 51 nucleotides BPHYT_RS20450 and BPHYT_RS20455 are separated by 108 nucleotides
BPHYT_RS20445: BPHYT_RS20445 - NAD-dependent dehydratase, at 149,568 to 150,632
_RS20445
BPHYT_RS20450: BPHYT_RS20450 - 6-chlorohydroxyquinol-1,2-dioxygenase, at 150,684 to 151,547
_RS20450
BPHYT_RS20455: BPHYT_RS20455 - hypothetical protein, at 151,656 to 152,333
_RS20455
Position (kb) 150
151
152 Strain fitness (log2 ratio) -1
0
1 at 150.067 kb on + strand, within BPHYT_RS20445 at 150.068 kb on - strand, within BPHYT_RS20445 at 150.196 kb on + strand, within BPHYT_RS20445 at 150.196 kb on + strand, within BPHYT_RS20445 at 150.197 kb on - strand, within BPHYT_RS20445 at 150.199 kb on + strand, within BPHYT_RS20445 at 150.199 kb on + strand, within BPHYT_RS20445 at 150.290 kb on - strand, within BPHYT_RS20445 at 150.290 kb on - strand, within BPHYT_RS20445 at 150.638 kb on + strand at 150.638 kb on + strand at 150.874 kb on + strand, within BPHYT_RS20450 at 150.944 kb on + strand, within BPHYT_RS20450 at 151.078 kb on + strand, within BPHYT_RS20450 at 151.079 kb on - strand, within BPHYT_RS20450 at 151.079 kb on - strand, within BPHYT_RS20450 at 151.664 kb on + strand at 151.878 kb on - strand, within BPHYT_RS20455 at 152.018 kb on - strand, within BPHYT_RS20455 at 152.018 kb on - strand, within BPHYT_RS20455 at 152.058 kb on - strand, within BPHYT_RS20455 at 152.142 kb on - strand, within BPHYT_RS20455 at 152.153 kb on + strand, within BPHYT_RS20455 at 152.167 kb on + strand, within BPHYT_RS20455 at 152.423 kb on + strand at 152.475 kb on + strand at 152.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO9 MOI 1 remove 150,067 + BPHYT_RS20445 0.47 -0.1 150,068 - BPHYT_RS20445 0.47 +0.3 150,196 + BPHYT_RS20445 0.59 +0.5 150,196 + BPHYT_RS20445 0.59 +1.7 150,197 - BPHYT_RS20445 0.59 +0.8 150,199 + BPHYT_RS20445 0.59 +0.6 150,199 + BPHYT_RS20445 0.59 -1.4 150,290 - BPHYT_RS20445 0.68 -0.5 150,290 - BPHYT_RS20445 0.68 -1.0 150,638 + -0.5 150,638 + -0.9 150,874 + BPHYT_RS20450 0.22 +0.1 150,944 + BPHYT_RS20450 0.30 +0.2 151,078 + BPHYT_RS20450 0.46 +1.0 151,079 - BPHYT_RS20450 0.46 +0.4 151,079 - BPHYT_RS20450 0.46 -1.6 151,664 + +1.5 151,878 - BPHYT_RS20455 0.33 -0.3 152,018 - BPHYT_RS20455 0.53 +0.1 152,018 - BPHYT_RS20455 0.53 -0.2 152,058 - BPHYT_RS20455 0.59 -0.3 152,142 - BPHYT_RS20455 0.72 +1.0 152,153 + BPHYT_RS20455 0.73 -0.6 152,167 + BPHYT_RS20455 0.75 +0.2 152,423 + +0.3 152,475 + -0.3 152,517 - +0.2
Or see this region's nucleotide sequence